BRENDA - Enzyme Database
show all sequences of 2.8.3.9

Purification and properties of Escherichia coli coenzyme A-transferase

Sramek, S.J.; Frerman, F.E.; Arch. Biochem. Biophys. 171, 14-26 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
epsilon-Acetyl-CoA
-
Escherichia coli
additional information
partially inactivated by acyl-CoA substrates in absence of carboxylic acid substrates
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
acetyl-CoA
pH 8.1, 24°C
Escherichia coli
6.7
-
epsilon-Acetyl-CoA
pH 8.1, 24°C
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Escherichia coli
16020
-
spheroplast
-
Escherichia coli
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
26000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
97800
-
sedimentation equilibrium centrifugation
Escherichia coli
98000
-
SDS-PAGE
Escherichia coli
99000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoate + acetoacetyl-CoA
Escherichia coli
-
butanoyl-CoA + acetoacetate
-
Escherichia coli
r
butanoate + acetoacetyl-CoA
Escherichia coli C22
-
butanoyl-CoA + acetoacetate
-
Escherichia coli C22
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
C22
-
Escherichia coli C22
-
C22
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
contains no detectable carbohydrate
Escherichia coli
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
117
-
fraction B
Escherichia coli
160
-
fraction A
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-pentenoate + acetoacetyl-CoA
reaction at 21% the rate of acetate
643837
Escherichia coli
4-pentenoyl-CoA + acetoacetate
-
-
-
r
acetate + acetoacetyl-CoA
best substrates
643837
Escherichia coli
acetyl-CoA + acetoacetate
-
643837
Escherichia coli
r
acetate + acetoacetyl-CoA
best substrates
643837
Escherichia coli C22
acetyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
acetyl-CoA + acetoacetate
best substrates
643837
Escherichia coli
acetate + acetoacetyl-CoA
-
643837
Escherichia coli
r
acetyl-CoA + acetoacetate
best substrates
643837
Escherichia coli C22
acetate + acetoacetyl-CoA
-
643837
Escherichia coli C22
r
butanoate + acetoacetyl-CoA
-
643837
Escherichia coli
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli
r
butanoate + acetoacetyl-CoA
reaction at 68% the rate of acetate
643837
Escherichia coli
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli
r
butanoate + acetoacetyl-CoA
-
643837
Escherichia coli C22
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
butanoate + acetoacetyl-CoA
reaction at 68% the rate of acetate
643837
Escherichia coli C22
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
butyrate + acetyl-CoA
reaction at 56% the rate of acetoacetate, reverse reaction at 86% the rate of acetoacetyl-CoA
643837
Escherichia coli
butyryl-CoA + acetate
-
643837
Escherichia coli
r
crotonyl-CoA + acetate
reaction at 4% the rate of acetoacetylCoA
643837
Escherichia coli
crotonate + acetyl-CoA
-
643837
Escherichia coli
r
DL-3-hydroxybutyrate + acetyl-CoA
reaction at 9% the rate of acetoacetate
643837
Escherichia coli
3-hydroxybutyryl-CoA + acetate
-
-
-
r
epsilon-acetyl-CoA + acetoacetate
reaction at 1% the rate of acetylCoA
643837
Escherichia coli
acetate + acetoacetyl-CoA
-
643837
Escherichia coli
r
formate + acetoacetyl-CoA
reaction at 7% the rate of acetate
643837
Escherichia coli
formyl-CoA + acetoacetate
-
-
-
r
additional information
decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates
643837
Escherichia coli
?
-
-
-
-
additional information
decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates
643837
Escherichia coli C22
?
-
-
-
-
octanoate + acetoacetyl-CoA
reaction at 3% the rate of acetate
643837
Escherichia coli
octanoyl-CoA + acetoacetate
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10.6
-
epsilon-Acetyl-CoA
pH 8.1, 24°C
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
epsilon-Acetyl-CoA
-
Escherichia coli
additional information
partially inactivated by acyl-CoA substrates in absence of carboxylic acid substrates
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10.6
-
epsilon-Acetyl-CoA
pH 8.1, 24°C
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
acetyl-CoA
pH 8.1, 24°C
Escherichia coli
6.7
-
epsilon-Acetyl-CoA
pH 8.1, 24°C
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Escherichia coli
16020
-
spheroplast
-
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
26000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
97800
-
sedimentation equilibrium centrifugation
Escherichia coli
98000
-
SDS-PAGE
Escherichia coli
99000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoate + acetoacetyl-CoA
Escherichia coli
-
butanoyl-CoA + acetoacetate
-
Escherichia coli
r
butanoate + acetoacetyl-CoA
Escherichia coli C22
-
butanoyl-CoA + acetoacetate
-
Escherichia coli C22
r
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
contains no detectable carbohydrate
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
117
-
fraction B
Escherichia coli
160
-
fraction A
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-pentenoate + acetoacetyl-CoA
reaction at 21% the rate of acetate
643837
Escherichia coli
4-pentenoyl-CoA + acetoacetate
-
-
-
r
acetate + acetoacetyl-CoA
best substrates
643837
Escherichia coli
acetyl-CoA + acetoacetate
-
643837
Escherichia coli
r
acetate + acetoacetyl-CoA
best substrates
643837
Escherichia coli C22
acetyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
acetyl-CoA + acetoacetate
best substrates
643837
Escherichia coli
acetate + acetoacetyl-CoA
-
643837
Escherichia coli
r
acetyl-CoA + acetoacetate
best substrates
643837
Escherichia coli C22
acetate + acetoacetyl-CoA
-
643837
Escherichia coli C22
r
butanoate + acetoacetyl-CoA
-
643837
Escherichia coli
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli
r
butanoate + acetoacetyl-CoA
reaction at 68% the rate of acetate
643837
Escherichia coli
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli
r
butanoate + acetoacetyl-CoA
-
643837
Escherichia coli C22
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
butanoate + acetoacetyl-CoA
reaction at 68% the rate of acetate
643837
Escherichia coli C22
butanoyl-CoA + acetoacetate
-
643837
Escherichia coli C22
r
butyrate + acetyl-CoA
reaction at 56% the rate of acetoacetate, reverse reaction at 86% the rate of acetoacetyl-CoA
643837
Escherichia coli
butyryl-CoA + acetate
-
643837
Escherichia coli
r
crotonyl-CoA + acetate
reaction at 4% the rate of acetoacetylCoA
643837
Escherichia coli
crotonate + acetyl-CoA
-
643837
Escherichia coli
r
DL-3-hydroxybutyrate + acetyl-CoA
reaction at 9% the rate of acetoacetate
643837
Escherichia coli
3-hydroxybutyryl-CoA + acetate
-
-
-
r
epsilon-acetyl-CoA + acetoacetate
reaction at 1% the rate of acetylCoA
643837
Escherichia coli
acetate + acetoacetyl-CoA
-
643837
Escherichia coli
r
formate + acetoacetyl-CoA
reaction at 7% the rate of acetate
643837
Escherichia coli
formyl-CoA + acetoacetate
-
-
-
r
additional information
decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates
643837
Escherichia coli
?
-
-
-
-
additional information
decanoyl-CoA and succinyl-CoA cannot replace acetoacetyl-CoA, succinate, malonate and 2,3-butandienoate cannot replace acetate, 3-carbon dicarboxylic and 4-carbon dicarboxylic acids are completely inactive as substrates
643837
Escherichia coli C22
?
-
-
-
-
octanoate + acetoacetyl-CoA
reaction at 3% the rate of acetate
643837
Escherichia coli
octanoyl-CoA + acetoacetate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
Other publictions for EC 2.8.3.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737542
Yu
Metabolic engineering of Clost ...
Clostridium tyrobutyricum
Appl. Microbiol. Biotechnol.
99
4917-4930
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643839
Emmanuel
-
Butyrate:acetoacetyl-CoA trans ...
Bos taurus
Can. J. Anim. Sci.
63
355-360
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643838
Barker
Butyryl-CoA:acetoacetate CoA-t ...
Acetoanaerobium sticklandii, Clostridium sp., Clostridium sp. SB4
J. Biol. Chem.
253
1219-1225
1978
1
-
-
-
-
1
-
30
-
7
4
3
-
14
-
-
1
-
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
30
-
7
4
3
-
-
-
1
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
643836
Sramek
Escherichia coli coenzyme A-tr ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
27-35
1975
-
-
-
-
-
-
2
2
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643837
Sramek
Purification and properties of ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
14-26
1975
-
-
-
-
-
-
3
2
2
-
5
2
-
3
-
1
1
-
-
-
2
-
17
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
2
2
-
5
2
-
-
1
1
-
-
2
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-