BRENDA - Enzyme Database
show all sequences of 2.8.3.9

Escherichia coli coenzyme A-transferase: kinetics, catalytic pathway and structure

Sramek, S.J.; Frerman, F.E.; Arch. Biochem. Biophys. 171, 27-35 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
epsilon-Acetyl-CoA
-
Escherichia coli
NaBH4
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
acetoacetyl-CoA
pH 8.1, 25°C
Escherichia coli
0.26
-
acetyl-CoA
pH 8.1, 25°C
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
26000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
97000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
C22
-
Escherichia coli C22
-
C22
-
Subunits
Subunits
Commentary
Organism
tetramer
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10.6
-
epsilon-Acetyl-CoA
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
epsilon-Acetyl-CoA
-
Escherichia coli
NaBH4
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10.6
-
epsilon-Acetyl-CoA
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
acetoacetyl-CoA
pH 8.1, 25°C
Escherichia coli
0.26
-
acetyl-CoA
pH 8.1, 25°C
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
26000
-
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
97000
-
gel filtration
Escherichia coli
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 23000 + 2 * 26000, 2 alpha and 2 beta subunits, SDS-PAGE
Escherichia coli
Other publictions for EC 2.8.3.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737542
Yu
Metabolic engineering of Clost ...
Clostridium tyrobutyricum
Appl. Microbiol. Biotechnol.
99
4917-4930
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643839
Emmanuel
-
Butyrate:acetoacetyl-CoA trans ...
Bos taurus
Can. J. Anim. Sci.
63
355-360
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643838
Barker
Butyryl-CoA:acetoacetate CoA-t ...
Acetoanaerobium sticklandii, Clostridium sp., Clostridium sp. SB4
J. Biol. Chem.
253
1219-1225
1978
1
-
-
-
-
1
-
30
-
7
4
3
-
14
-
-
1
-
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
30
-
7
4
3
-
-
-
1
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
643836
Sramek
Escherichia coli coenzyme A-tr ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
27-35
1975
-
-
-
-
-
-
2
2
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643837
Sramek
Purification and properties of ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
14-26
1975
-
-
-
-
-
-
3
2
2
-
5
2
-
3
-
1
1
-
-
-
2
-
17
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
2
2
-
5
2
-
-
1
1
-
-
2
-
17
1
-
-
-
-
-
-
-
-
-
-
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-
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