BRENDA - Enzyme Database show
show all sequences of 2.8.3.8

Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases

Mullins, E.A.; Kappock, T.J.; Biochemistry 51, 8422-8434 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Acetobacter aceti
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.7-2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate (pH 6.5), and 25 mM 2-mercaptoethanol
Acetobacter aceti
Engineering
Amino acid exchange
Commentary
Organism
E294A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
Acetobacter aceti
E435A
insoluble mutant
Acetobacter aceti
E435D
the mutant shows almost wild type activity
Acetobacter aceti
E435Q
insoluble mutant
Acetobacter aceti
R228E
the mutant has a specific defect in its ability to bind both carboxylate substrates succinyl-CoA and acetyl-CoA and does not use 4-aminobutyrate as substrate
Acetobacter aceti
S71A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
Acetobacter aceti
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
-
Acetobacter aceti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
110
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter aceti
B3EY95
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, dye-affinity chromatography, ion-exchange column chromatography, and gel filtration
Acetobacter aceti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + succinyl-CoA
5.1% activity compared to succinate
721675
Acetobacter aceti
succinate + acetyl-CoA
-
-
-
r
acetate + succinyl-CoA
5.1% activity compared to succinate
721675
Acetobacter aceti 1023
succinate + acetyl-CoA
-
-
-
r
additional information
less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate
721675
Acetobacter aceti
?
-
-
-
-
additional information
less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate
721675
Acetobacter aceti 1023
?
-
-
-
-
succinate + acetyl-CoA
100% activity
721675
Acetobacter aceti
acetate + succinyl-CoA
-
-
-
r
succinate + acetyl-CoA
100% activity
721675
Acetobacter aceti 1023
acetate + succinyl-CoA
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
290
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1600
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Acetobacter aceti
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.7-2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate (pH 6.5), and 25 mM 2-mercaptoethanol
Acetobacter aceti
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E294A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
Acetobacter aceti
E435A
insoluble mutant
Acetobacter aceti
E435D
the mutant shows almost wild type activity
Acetobacter aceti
E435Q
insoluble mutant
Acetobacter aceti
R228E
the mutant has a specific defect in its ability to bind both carboxylate substrates succinyl-CoA and acetyl-CoA and does not use 4-aminobutyrate as substrate
Acetobacter aceti
S71A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
Acetobacter aceti
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
-
Acetobacter aceti
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1600
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
110
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, dye-affinity chromatography, ion-exchange column chromatography, and gel filtration
Acetobacter aceti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + succinyl-CoA
5.1% activity compared to succinate
721675
Acetobacter aceti
succinate + acetyl-CoA
-
-
-
r
acetate + succinyl-CoA
5.1% activity compared to succinate
721675
Acetobacter aceti 1023
succinate + acetyl-CoA
-
-
-
r
additional information
less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate
721675
Acetobacter aceti
?
-
-
-
-
additional information
less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate
721675
Acetobacter aceti 1023
?
-
-
-
-
succinate + acetyl-CoA
100% activity
721675
Acetobacter aceti
acetate + succinyl-CoA
-
-
-
r
succinate + acetyl-CoA
100% activity
721675
Acetobacter aceti 1023
acetate + succinyl-CoA
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
290
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
General Information
General Information
Commentary
Organism
metabolism
succinyl-CoA:acetate CoA-transferase is an acetic acid resistance factor (AarC) with a role in a variant citric acid cycle
Acetobacter aceti
physiological function
the enzyme is an acetic acid resistance factor that is required for acetate resistance
Acetobacter aceti
General Information (protein specific)
General Information
Commentary
Organism
metabolism
succinyl-CoA:acetate CoA-transferase is an acetic acid resistance factor (AarC) with a role in a variant citric acid cycle
Acetobacter aceti
physiological function
the enzyme is an acetic acid resistance factor that is required for acetate resistance
Acetobacter aceti
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.029
-
acetate
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.03
-
succinate
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.074
-
succinyl-CoA
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.13
-
succinyl-CoA
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.16
-
acetate
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.3
-
succinate
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.39
-
succinyl-CoA
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.6
-
acetate
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.7
-
acetyl-CoA
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C; mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2.3
-
acetyl-CoA
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2.7
-
succinate
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
3
-
acetate
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
3.4
-
acetyl-CoA
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
9
-
succinyl-CoA
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C; wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
52
-
acetyl-CoA
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
73
-
succinate
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
79
-
succinate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2600
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.029
-
acetate
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.03
-
succinate
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.074
-
succinyl-CoA
mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.13
-
succinyl-CoA
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.16
-
acetate
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.3
-
succinate
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.39
-
succinyl-CoA
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.6
-
acetate
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
0.7
-
acetyl-CoA
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C; mutant enzyme S71A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2.3
-
acetyl-CoA
mutant enzyme R228E, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2.7
-
succinate
mutant enzyme N347A, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
3
-
acetate
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
3.4
-
acetyl-CoA
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
9
-
succinyl-CoA
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C; wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
52
-
acetyl-CoA
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
73
-
succinate
mutant enzyme E435D, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
79
-
succinate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
2600
-
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
Acetobacter aceti
Other publictions for EC 2.8.3.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737509
Trachsel
Function and phylogeny of bact ...
Butyricicoccus porcorum, Eubacterium nodatum, Eubacterium nodatum 68-3-10, Megasphaera elsdenii 14-14, Megasphaera elsdenii
Appl. Environ. Microbiol.
82
6788-6798
2016
-
-
3
-
-
-
-
-
-
-
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5
-
5
-
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-
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-
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5
-
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3
-
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5
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5
-
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-
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-
-
-
-
-
-
737906
Shaw
Anaerobic detoxification of ac ...
Thermoanaerobacterium saccharolyticum, Thermoanaerobacterium saccharolyticum M2212
Biotechnol. Biofuels
8
75
2015
-
-
-
-
-
-
-
-
-
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2
-
4
-
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-
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2
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2
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2
-
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-
-
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-
-
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-
738737
Deng
Driving carbon flux through ex ...
Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
J. Biotechnol.
216
151-157
2015
-
-
1
-
-
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-
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2
-
7
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-
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2
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1
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2
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2
-
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-
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-
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-
721675
Mullins
Crystal structures of Acetobac ...
Acetobacter aceti
Biochemistry
51
8422-8434
2012
-
-
1
1
6
-
1
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
6
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
2
2
-
18
18
725472
Millerioux
ATP synthesis-coupled and -unc ...
Trypanosoma brucei
J. Biol. Chem.
287
17186-17197
2012
-
-
-
-
-
-
-
-
2
-
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2
-
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1
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2
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1
-
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-
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-
-
2
2
-
-
-
724855
Louis
Diversity of human colonic but ...
Anaerostipes caccae, Anaerostipes caccae DSM 14662, Butyrivibrio fibrisolvens 16/4, Butyrivibrio fibrisolvens, Eubacterium hallii, Eubacterium hallii M72/1, Eubacterium rectale, Eubacterium rectale DSM 17629, Faecalibacterium prausnitzii, Faecalibacterium prausnitzii A2-165, Faecalibacterium prausnitzii L2-6, Faecalibacterium prausnitzii M21/2, Roseburia intestinalis, Roseburia intestinalis L1-82, Roseburia inulinivorans, Roseburia inulinivorans A2-194
Environ. Microbiol.
12
304-314
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
2
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690517
Louis
Development of a semiquantitat ...
Anaerostipes caccae, Eubacterium hallii, Faecalibacterium prausnitzii, Roseburia hominis, Roseburia hominis A2-183
Appl. Environ. Microbiol.
73
2009-2012
2007
-
4
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
-
4
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646005
Duncan
Acetate utilization and butyry ...
Coprococcus sp., Coprococcus sp. L2-50, Faecalibacterium prausnitzii, Roseburia intestinalis, Roseburia intestinalis L1-82, Roseburia sp., Roseburia sp. A2-181
Appl. Environ. Microbiol.
68
5186-5190
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
14
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646006
Korolev
Autotracing of Escherichia col ...
Escherichia coli
Acta Crystallogr. Sect. D
58
2116-2121
2002
-
-
1
1
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
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1
-
1
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1
-
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-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646004
Vanderwinkel
Growth of Escherichia coli on ...
Escherichia coli
Biochem. Biophys. Res. Commun.
33
902-908
1968
-
-
-
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