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Literature summary for 2.8.3.6 extracted from

  • Yeh, W.K.; Ornston, L.N.
    p-Chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl CoA: beta-ketoadipate CoA transferase (1984), Arch. Microbiol., 138, 102-105.
    View publication on PubMed
Search for more literature for 2.8.3.6

General Stability

General Stability Organism
stable for 1h at 22°C in the presence of 0.00092 mM DTT Acinetobacter calcoaceticus
unstable in the absence of sulfhydryl compounds, DTT stabilizes, 80% residual activity after 5h at 0°C in 0.00086 mM DTT Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores Acinetobacter calcoaceticus
4-chloromercuribenzoate modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 mutant strain ADP152, 2 transferases, I: induced by protocatechuate, II: induced by cis,cis-muconate
-
Pseudomonas putida
-
-
-

Storage Stability

Storage Stability Organism
0°C, in the presence of DTT, 20% loss of activity within 5 h Pseudomonas putida
0°C, in the presence of DTT, 20% loss of activity within 5 h Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + 3-oxoadipate
-
 Pseudomonas putida succinate + 3-oxoadipyl-CoA
-
 ?
succinyl-CoA + 3-oxoadipate
-
 Acinetobacter calcoaceticus succinate + 3-oxoadipyl-CoA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 1 h, stable in the presence of DTT Acinetobacter calcoaceticus