BRENDA - Enzyme Database
show all sequences of 2.8.3.3

Purification and some properties of malonate decarboxylase from Pseudomonas ovalis: an oligomeric enzyme with bifunctional properties

Takamura, Y.; Kitayama, Y.; Biochem. Int. 3, 483-491 (1981)
No PubMed abstract available

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
40000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
70000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonate
Pseudomonas putida
inducible enzyme
acetate + malonyl-CoA
-
-
?
acetyl-CoA + malonate
Pseudomonas putida IAM 1177
inducible enzyme
acetate + malonyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
IAM 1177
-
Pseudomonas putida IAM 1177
-
IAM 1177
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida
acetate + malonyl-CoA
-
4864
Pseudomonas putida
?
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida
acetate + malonyl-CoA
-
-
-
?
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
?
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
-
-
?
butyryl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + butanoate
-
-
-
?
malonyl-CoA + malonate
exchange reaction
4864
Pseudomonas putida
malonyl-CoA + malonate
-
-
-
?
methylmalonyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + methylmalonate
-
-
-
?
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida
?
-
-
-
-
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida IAM 1177
?
-
-
-
-
propionyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + propanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
40000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
70000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonate
Pseudomonas putida
inducible enzyme
acetate + malonyl-CoA
-
-
?
acetyl-CoA + malonate
Pseudomonas putida IAM 1177
inducible enzyme
acetate + malonyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida
acetate + malonyl-CoA
-
4864
Pseudomonas putida
?
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida
acetate + malonyl-CoA
-
-
-
?
acetyl-CoA + malonate
absolutely dependent on acetyl-CoA
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
4864
Pseudomonas putida IAM 1177
?
acetyl-CoA + malonate
inducible enzyme
4864
Pseudomonas putida IAM 1177
acetate + malonyl-CoA
-
-
-
?
butyryl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + butanoate
-
-
-
?
malonyl-CoA + malonate
exchange reaction
4864
Pseudomonas putida
malonyl-CoA + malonate
-
-
-
?
methylmalonyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + methylmalonate
-
-
-
?
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida
?
-
-
-
-
additional information
succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA
4864
Pseudomonas putida IAM 1177
?
-
-
-
-
propionyl-CoA + malonate
-
4864
Pseudomonas putida
malonyl-CoA + propanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
Other publictions for EC 2.8.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
4864
Takamura
-
Purification and some properti ...
Pseudomonas putida, Pseudomonas putida IAM 1177
Biochem. Int.
3
483-491
1981
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4
2
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2
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1
-
-
-
-
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10
1
1
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
4
2
-
-
-
1
-
-
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
645966
Hayaishi
Enzymatic decarboxylation of m ...
Pseudomonas fluorescens, Pseudomonas fluorescens 23
J. Biol. Chem.
215
125-136
1955
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3
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2
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2
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