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Literature summary for 2.8.3.22 extracted from

  • Friedmann, S.; Steindorf, A.; Alber, B.E.; Fuchs, G.
    Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2006), J. Bacteriol., 188, 2646-2655.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.22

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Chloroflexus aurantiacus

Inhibitors

Inhibitors Comment Organism Structure
Borohydride no inactivation by 0.1 mM borohydride is recorded in the test with and without succinyl-CoA, and nearly 85% of the activity is retained when the enzyme is preincubated with 10 mM hydroxylamine for 10 min in the presence of 2 mM succinyl-CoA. This suggests that no reactive enzyme-CoA intermediate is present. Inactivation with 10 mM borohydride decreases activity to 28% in the presence of 2 mM succinyl-CoA Chloroflexus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 succinyl-CoA 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
1.1
-
 (S)-citramalate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
1.3
-
 (S)-malate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6000
-
 x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus
44000
-
 x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus
550000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + (S)-malate Chloroflexus aurantiacus the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation succinate + (S)-malyl-CoA
-
 r
succinyl-CoA + (S)-malate Chloroflexus aurantiacus DSM Z 636 the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation succinate + (S)-malyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-
Chloroflexus aurantiacus DSM Z 636
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chloroflexus aurantiacus

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + (S)-citramalate = succinate + (S)-citramalyl-CoA (2) Chloroflexus aurantiacus
a
succinyl-CoA + (S)-malate = succinate + (S)-malyl-CoA (1) Chloroflexus aurantiacus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

Storage Stability

Storage Stability Organism
4°C, Tris buffer, pH 7, containing 100 mM KCl, 1 week, stable Chloroflexus aurantiacus
frozen, stable for several weeks with 10% glycerol (v/v) Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + (S)-citramalate 2fold higher activity than with (S)-malate Chloroflexus aurantiacus succinate + (S)-citramalyl-CoA
-
 ?
succinyl-CoA + (S)-citramalate 2fold higher activity than with (S)-malate Chloroflexus aurantiacus DSM Z 636 succinate + (S)-citramalyl-CoA
-
 ?
succinyl-CoA + (S)-malate the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus succinate + (S)-malyl-CoA
-
 r
succinyl-CoA + (S)-malate no activity with (R)-malate. Succinyl-CoA is the only CoA donor found so far which could not be substituted by propionyl-CoA, malonyl-CoA, or acetyl-CoA Chloroflexus aurantiacus succinate + (S)-malyl-CoA
-
 r
succinyl-CoA + (S)-malate the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus DSM Z 636 succinate + (S)-malyl-CoA
-
 r
succinyl-CoA + (S)-malate no activity with (R)-malate. Succinyl-CoA is the only CoA donor found so far which could not be substituted by propionyl-CoA, malonyl-CoA, or acetyl-CoA Chloroflexus aurantiacus DSM Z 636 succinate + (S)-malyl-CoA
-
 r
succinyl-CoA + itaconate 12% of the activity compared to (S)-malate Chloroflexus aurantiacus succinate + ?
-
 ?
succinyl-CoA + itaconate 12% of the activity compared to (S)-malate Chloroflexus aurantiacus DSM Z 636 succinate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
SmtAB
-
 Chloroflexus aurantiacus
succinyl-CoA:L-malate coenzyme A transferase
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.4
-
 (S)-malate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
11.4
-
 succinyl-CoA 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Chloroflexus aurantiacus

General Information

General Information Comment Organism
physiological function the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus