Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus

Friedmann, S.; Alber, B.E.; Fuchs, G.; J. Bacteriol. 188, 6460-6468 (2006)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.08

-

succinyl-CoA

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

2.2

-

(R)-citramalate

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus DSM 636

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus OK-70-fl / DSM 636

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

Reaction

Commentary

Organism

Reaction ID

succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA

(1)

Chloroflexus aurantiacus

succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA

(2)

Chloroflexus aurantiacus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus

succinate + ?

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus DSM 636

succinate + ?

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + ?

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

13

-

(R)-citramalate

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

13

-

succinyl-CoA

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.08

-

succinyl-CoA

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

2.2

-

(R)-citramalate

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus DSM 636

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

succinyl-CoA + (R)-citramalate

Chloroflexus aurantiacus OK-70-fl / DSM 636

the enzyme catalyzes a steps in the glyoxylate assimilation route

succinate + (R)-citramalyl-CoA

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

-

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-citramalate

the enzyme catalyzes a steps in the glyoxylate assimilation route

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-citramalyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus DSM 636

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + (R)-malate

88% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + (R)-malyl-CoA

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus

succinate + ?

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus DSM 636

succinate + ?

-

-

-

?

succinyl-CoA + itaconate

80% compared to the activity with (R)-citramalate

674298

Chloroflexus aurantiacus OK-70-fl / DSM 636

succinate + ?

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

13

-

(R)-citramalate

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

13

-

succinyl-CoA

55°C, pH and temperature not specified in the publication

Chloroflexus aurantiacus

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

674298

Friedmann

Properties of succinyl-coenzym ...

Chloroflexus aurantiacus, Chloroflexus aurantiacus DSM 636

J. Bacteriol.

188

6460-6468

2006

-

-

1

-

-

-

-

2

-

1

2

3

-

3

-

-

1

2

-

-

1

2

12

1

1

1

-

1

2

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

2

-

1

2

3

-

-

-

1

-

-

1

2

12

1

1

-

1

2

1

-

-

-

1

1

1

1

-

-