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show all sequences of 2.8.3.20

Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus

Friedmann, S.; Alber, B.E.; Fuchs, G.; J. Bacteriol. 188, 6460-6468 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologously expressed in Escherichia coli
Chloroflexus aurantiacus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
succinyl-CoA
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
2.2
-
(R)-citramalate
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
activity is not dependent on Mg2+
Chloroflexus aurantiacus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
90000
-
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus OK-70-fl / DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chloroflexus aurantiacus
-
-
-
Chloroflexus aurantiacus DSM 636
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Chloroflexus aurantiacus
Reaction
Reaction
Commentary
Organism
succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA
(1)
Chloroflexus aurantiacus
succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA
(2)
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
17.5
-
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Storage Stability
Storage Stability
Organism
4C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity
Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity
Chloroflexus aurantiacus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + ?
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + ?
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Chloroflexus aurantiacus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
stable
Chloroflexus aurantiacus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13
-
(R)-citramalate
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
13
-
succinyl-CoA
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
at 55C
Chloroflexus aurantiacus
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologously expressed in Escherichia coli
Chloroflexus aurantiacus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
succinyl-CoA
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
2.2
-
(R)-citramalate
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
activity is not dependent on Mg2+
Chloroflexus aurantiacus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
90000
-
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus OK-70-fl / DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
17.5
-
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Storage Stability (protein specific)
Storage Stability
Organism
4C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity
Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity
Chloroflexus aurantiacus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
-
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-malyl-CoA
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + ?
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + ?
-
-
-
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Chloroflexus aurantiacus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
stable
Chloroflexus aurantiacus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13
-
(R)-citramalate
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
13
-
succinyl-CoA
55C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
at 55C
Chloroflexus aurantiacus
Expression
Organism
Commentary
Expression
Chloroflexus aurantiacus
upregulated under autotrophic conditions
up
General Information
General Information
Commentary
Organism
physiological function
the enzyme catalyzes a steps in the glyoxylate assimilation route
Chloroflexus aurantiacus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme catalyzes a steps in the glyoxylate assimilation route
Chloroflexus aurantiacus
Expression (protein specific)
Organism
Commentary
Expression
Chloroflexus aurantiacus
upregulated under autotrophic conditions
up
Other publictions for EC 2.8.3.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674298
Friedmann
Properties of succinyl-coenzym ...
Chloroflexus aurantiacus, Chloroflexus aurantiacus DSM 636
J. Bacteriol.
188
6460-6468
2006
-
-
1
-
-
-
-
2
-
1
2
3
-
3
-
-
1
2
-
-
1
2
12
1
1
-
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
2
3
-
-
-
1
-
-
1
2
12
1
1
-
1
2
1
-
-
-
1
1
1
1
-
-