Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Chloroflexus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
succinyl-CoA | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus | |
2.2 | - |
(R)-citramalate | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is not dependent on Mg2+ | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Chloroflexus aurantiacus |
90000 | - |
gel filtration | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus DSM 636 | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | the enzyme catalyzes a steps in the glyoxylate assimilation route | succinate + (R)-citramalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
- |
- |
Chloroflexus aurantiacus DSM 636 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Chloroflexus aurantiacus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA | (1) | Chloroflexus aurantiacus | |
succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA | (2) | Chloroflexus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.5 | - |
55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
Storage Stability | Organism |
---|---|
4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity | Chloroflexus aurantiacus |
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-citramalate | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-citramalyl-CoA | - |
? | |
succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus | succinate + (R)-malyl-CoA | - |
? | |
succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus DSM 636 | succinate + (R)-malyl-CoA | - |
? | |
succinyl-CoA + (R)-malate | 88% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + (R)-malyl-CoA | - |
? | |
succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus | succinate + ? | - |
? | |
succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus DSM 636 | succinate + ? | - |
? | |
succinyl-CoA + itaconate | 80% compared to the activity with (R)-citramalate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | succinate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 44000, SDS-PAGE | Chloroflexus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
SCT | - |
Chloroflexus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Chloroflexus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable | Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
succinyl-CoA | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus | |
13 | - |
(R)-citramalate | 55°C, pH and temperature not specified in the publication | Chloroflexus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
at 55°C | Chloroflexus aurantiacus |
Organism | Comment | Expression |
---|---|---|
Chloroflexus aurantiacus | upregulated under autotrophic conditions | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes a steps in the glyoxylate assimilation route | Chloroflexus aurantiacus |