Data extracted from this reference:
Cloned(Commentary)
heterologously expressed in Escherichia coli
Chloroflexus aurantiacus
KM Value [mM]
0.08
succinyl-CoA
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
2.2
(R)-citramalate
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Metals/Ions
additional information
activity is not dependent on Mg2+
Chloroflexus aurantiacus
Molecular Weight [Da]
44000
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
90000
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates)
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus OK-70-fl / DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
Organism
Chloroflexus aurantiacus DSM 636
Purification (Commentary)
partial
Chloroflexus aurantiacus
Reaction
succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA
(1)
Chloroflexus aurantiacus
succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA
(2)
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg]
17.5
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Storage Stability
4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity
Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity
Chloroflexus aurantiacus
Substrates and Products (Substrate)
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-malyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-malyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-malyl-CoA
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + ?
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + ?
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + ?
?
Subunits
homodimer
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
Synonyms
SCT
Chloroflexus aurantiacus
Temperature Optimum [°C]
55
Chloroflexus aurantiacus
Temperature Stability [°C]
55
stable
Chloroflexus aurantiacus
Turnover Number [1/s]
13
succinyl-CoA
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
13
(R)-citramalate
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
pH Optimum
6.5
at 55°C
Chloroflexus aurantiacus
Cloned(Commentary) (protein specific)
heterologously expressed in Escherichia coli
Chloroflexus aurantiacus
KM Value [mM] (protein specific)
0.08
succinyl-CoA
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
2.2
(R)-citramalate
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Metals/Ions (protein specific)
additional information
activity is not dependent on Mg2+
Chloroflexus aurantiacus
Molecular Weight [Da] (protein specific)
44000
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
90000
gel filtration
Chloroflexus aurantiacus
Natural Substrates/ Products (Substrates) (protein specific)
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
Chloroflexus aurantiacus OK-70-fl / DSM 636
the enzyme catalyzes a steps in the glyoxylate assimilation route
succinate + (R)-citramalyl-CoA
?
Purification (Commentary) (protein specific)
partial
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg] (protein specific)
17.5
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
Storage Stability (protein specific)
4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity
Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity
Chloroflexus aurantiacus
Substrates and Products (Substrate) (protein specific)
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-citramalate
the enzyme catalyzes a steps in the glyoxylate assimilation route
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-citramalyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + (R)-malyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + (R)-malyl-CoA
?
succinyl-CoA + (R)-malate
88% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + (R)-malyl-CoA
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus
succinate + ?
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus DSM 636
succinate + ?
?
succinyl-CoA + itaconate
80% compared to the activity with (R)-citramalate
674298
Chloroflexus aurantiacus OK-70-fl / DSM 636
succinate + ?
?
Subunits (protein specific)
homodimer
2 * 44000, SDS-PAGE
Chloroflexus aurantiacus
Temperature Optimum [°C] (protein specific)
55
Chloroflexus aurantiacus
Temperature Stability [°C] (protein specific)
55
stable
Chloroflexus aurantiacus
Turnover Number [1/s] (protein specific)
13
succinyl-CoA
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
13
(R)-citramalate
55°C, pH and temperature not specified in the publication
Chloroflexus aurantiacus
pH Optimum (protein specific)
6.5
at 55°C
Chloroflexus aurantiacus
Expression
Chloroflexus aurantiacus
upregulated under autotrophic conditions
up
General Information
physiological function
the enzyme catalyzes a steps in the glyoxylate assimilation route
Chloroflexus aurantiacus
General Information (protein specific)
physiological function
the enzyme catalyzes a steps in the glyoxylate assimilation route
Chloroflexus aurantiacus
Expression (protein specific)
Chloroflexus aurantiacus
upregulated under autotrophic conditions
up
Other publictions for EC 2.8.3.20
674298
Friedmann
Properties of succinyl-coenzym ...
Chloroflexus aurantiacus, Chloroflexus aurantiacus DSM 636
J. Bacteriol.
188
6460-6468
2006
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