BRENDA - Enzyme Database show
show all sequences of 2.8.3.18

Acetate:succinate CoA-transferase in the hydrogenosomes of Trichomonas vaginalis: identification and characterization

van Grinsven, K.W.; Rosnowsky, S.; van Weelden, S.W.; Puetz, S.; van der Giezen, M.; Martin, W.; van Hellemond, J.J.; Tielens, A.G.; Henze, K.; J. Biol. Chem. 283, 1411-1418 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in CHO cells
Trichomonas vaginalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
succinate
-
Trichomonas vaginalis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
hydrogenosome
-
Trichomonas vaginalis
42566
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
predicted from cDNA
Trichomonas vaginalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trichomonas vaginalis
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.007
-
specific activity of recombinant TvASCT expressed in CHO cells
Trichomonas vaginalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + acetyl-CoA
-
693049
Trichomonas vaginalis
acetate + succinyl-CoA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Trichomonas vaginalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Trichomonas vaginalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in CHO cells
Trichomonas vaginalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
succinate
-
Trichomonas vaginalis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
hydrogenosome
-
Trichomonas vaginalis
42566
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
predicted from cDNA
Trichomonas vaginalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.007
-
specific activity of recombinant TvASCT expressed in CHO cells
Trichomonas vaginalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + acetyl-CoA
-
693049
Trichomonas vaginalis
acetate + succinyl-CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Trichomonas vaginalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Trichomonas vaginalis
Other publictions for EC 2.8.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738287
Murphy
Functional dissection of the b ...
Acetobacter aceti 1023, Acetobacter aceti
Front. Chem.
4
23
2016
-
-
1
1
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721675
Mullins
Crystal structures of Acetobac ...
Acetobacter aceti 1023, Acetobacter aceti
Biochemistry
51
8422-8434
2012
-
-
1
1
7
-
3
1
-
-
-
1
-
4
-
-
-
1
-
-
-
-
20
-
1
-
-
-
1
-
2
-
3
-
-
-
-
1
-
1
7
-
-
3
3
1
-
-
-
1
-
-
-
-
-
-
-
-
20
-
1
-
-
-
1
-
2
-
-
3
3
-
25
25
692865
Mullins
A specialized citric acid cycl ...
Acetobacter aceti
J. Bacteriol.
190
4933-4940
2008
-
-
1
-
-
-
3
8
-
-
2
-
-
2
-
-
1
1
-
-
4
-
6
1
1
-
-
7
1
-
-
-
5
1
-
-
-
2
-
-
-
-
-
5
8
12
-
-
2
-
-
-
-
1
-
-
4
-
6
1
1
-
-
12
1
-
-
1
-
1
1
-
5
5
693049
van Grinsven
Acetate:succinate CoA-transfer ...
Trichomonas vaginalis
J. Biol. Chem.
283
1411-1418
2008
-
-
1
-
-
-
-
1
1
-
1
-
-
6
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662286
Riviere
Acetyl:succinate CoA-transfera ...
Trypanosoma brucei
J. Biol. Chem.
279
45337-45346
2004
-
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723135
Steinbüchel
Anaerobic pyruvate metabolism ...
Tritrichomonas foetus, Tritrichomonas foetus ATCC 30924
Mol. Biochem. Parasitol.
20
57-65
1986
-
-
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-