BRENDA - Enzyme Database show
show all sequences of 2.8.3.18

A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti

Mullins, E.A.; Francois, J.A.; Kappock, T.J.; J. Bacteriol. 190, 4933-4940 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a C-terminal hexahistidine-containing fusion peptide; expression in Escherichia coli
Acetobacter aceti
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
; substrate inhibition. Enzyme shows an increased substrate inhibition at a lower pH
Acetobacter aceti
dethiaacetyl-CoA
;
Acetobacter aceti
Sodium borohydride
-
Acetobacter aceti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
acetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
0.0221
-
succinyl-CoA
; pH 8.0, 25°C
Acetobacter aceti
0.0223
-
acetyl-CoA
-
Acetobacter aceti
0.9
-
succinate
; pH 8.0, 25°C
Acetobacter aceti
29
-
acetate
pH 5.0, 25°C
Acetobacter aceti
51
-
acetate
pH 6.0, 25°C
Acetobacter aceti
70
-
acetate
; pH 8.0, 25°C
Acetobacter aceti
130
-
acetoacetate
; pH 8.0, 25°C
Acetobacter aceti
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55847
-
3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein
Acetobacter aceti
160000
-
gel filtration, His-tagged recombinant protein
Acetobacter aceti
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter aceti
-
-
-
Acetobacter aceti
B3EY95
-
-
Purification (Commentary)
Commentary
Organism
using an ammonium sulfate fractionation procedure and an immobilized-metal affinity step. An 8-liter culture yields 9.5 mg of purified protein
Acetobacter aceti
Reaction
Reaction
Commentary
Organism
succinyl-CoA + acetate = acetyl-CoA + succinate
reaction displays ping-pong kinetics and a modified-enzyme mechanism
Acetobacter aceti
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
strain 2002, reverse reaction, pH 8.0
Acetobacter aceti
0.083
-
strain 1023, reverse reaction, pH 8.0
Acetobacter aceti
0.114
-
strain 2002, forward reaction, pH 8.0
Acetobacter aceti
0.17
-
strain 1023, forward reaction, pH 8.0
Acetobacter aceti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + succinyl-CoA
-
692865
Acetobacter aceti
succinate + acetyl-CoA
-
-
-
r
acetoacetate + succinyl-CoA
-
692865
Acetobacter aceti
succinate + acetoacetyl-CoA
-
-
-
r
acetyl-CoA + succinate
-
692865
Acetobacter aceti
succinyl-CoA + acetate
-
-
-
r
succinate + dethiaacetyl-CoA
-
692865
Acetobacter aceti
?
-
-
-
?
succinyl-CoA + acetate
-
692865
Acetobacter aceti
acetyl-CoA + succinate
-
-
-
r
succinyl-CoA + acetoacetate
-
692865
Acetobacter aceti
acetoacetyl-CoA + succinate
-
-
-
r
Subunits
Subunits
Commentary
Organism
trimer
3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein
Acetobacter aceti
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acetobacter aceti
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
36.5
-
acetoacetate
; pH 8.0, 25°C
Acetobacter aceti
49
-
acetate
pH 5.0, 25°C
Acetobacter aceti
70.9
-
succinate
; pH 8.0, 25°C
Acetobacter aceti
75.2
-
acetyl-CoA
; pH 8.0, 25°C
Acetobacter aceti
147
-
acetate
pH 6.0, 25°C
Acetobacter aceti
201
-
succinyl-CoA
; pH 8.0, 25°C
Acetobacter aceti
280
-
acetate
; pH 8.0, 25°C
Acetobacter aceti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Acetobacter aceti
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.016
-
dethiaacetyl-CoA
-
Acetobacter aceti
0.0166
-
dethiaacetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
500
-
acetate
pH 5.0, 25°C; pH: 5.0, shows an increased substrate inhibition at a lower pH
Acetobacter aceti
1100
-
acetate
pH 6.0, 25°C; pH: 6.0, shows an increased substrate inhibition at a lower pH
Acetobacter aceti
1600
-
acetate
pH: 8.0; pH 8.0, 25°C
Acetobacter aceti
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Acetobacter aceti
calculated
-
6.2
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a C-terminal hexahistidine-containing fusion peptide
Acetobacter aceti
expression in Escherichia coli
Acetobacter aceti
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
substrate inhibition. Enzyme shows an increased substrate inhibition at a lower pH
Acetobacter aceti
acetate
-
Acetobacter aceti
dethiaacetyl-CoA
-
Acetobacter aceti
Sodium borohydride
-
Acetobacter aceti
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.016
-
dethiaacetyl-CoA
-
Acetobacter aceti
0.0166
-
dethiaacetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
500
-
acetate
pH: 5.0, shows an increased substrate inhibition at a lower pH
Acetobacter aceti
500
-
acetate
pH 5.0, 25°C
Acetobacter aceti
1100
-
acetate
pH: 6.0, shows an increased substrate inhibition at a lower pH
Acetobacter aceti
1100
-
acetate
pH 6.0, 25°C
Acetobacter aceti
1600
-
acetate
pH: 8.0
Acetobacter aceti
1600
-
acetate
pH 8.0, 25°C
Acetobacter aceti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
acetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
0.0221
-
succinyl-CoA
-
Acetobacter aceti
0.0221
-
succinyl-CoA
pH 8.0, 25°C
Acetobacter aceti
0.0223
-
acetyl-CoA
-
Acetobacter aceti
0.9
-
succinate
-
Acetobacter aceti
0.9
-
succinate
pH 8.0, 25°C
Acetobacter aceti
29
-
acetate
pH 5.0, 25°C
Acetobacter aceti
51
-
acetate
pH 6.0, 25°C
Acetobacter aceti
70
-
acetate
-
Acetobacter aceti
70
-
acetate
pH 8.0, 25°C
Acetobacter aceti
130
-
acetoacetate
-
Acetobacter aceti
130
-
acetoacetate
pH 8.0, 25°C
Acetobacter aceti
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55847
-
3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein
Acetobacter aceti
160000
-
gel filtration, His-tagged recombinant protein
Acetobacter aceti
Purification (Commentary) (protein specific)
Commentary
Organism
using an ammonium sulfate fractionation procedure and an immobilized-metal affinity step. An 8-liter culture yields 9.5 mg of purified protein
Acetobacter aceti
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
strain 2002, reverse reaction, pH 8.0
Acetobacter aceti
0.083
-
strain 1023, reverse reaction, pH 8.0
Acetobacter aceti
0.114
-
strain 2002, forward reaction, pH 8.0
Acetobacter aceti
0.17
-
strain 1023, forward reaction, pH 8.0
Acetobacter aceti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetate + succinyl-CoA
-
692865
Acetobacter aceti
succinate + acetyl-CoA
-
-
-
r
acetoacetate + succinyl-CoA
-
692865
Acetobacter aceti
succinate + acetoacetyl-CoA
-
-
-
r
acetyl-CoA + succinate
-
692865
Acetobacter aceti
succinyl-CoA + acetate
-
-
-
r
succinate + dethiaacetyl-CoA
-
692865
Acetobacter aceti
?
-
-
-
?
succinyl-CoA + acetate
-
692865
Acetobacter aceti
acetyl-CoA + succinate
-
-
-
r
succinyl-CoA + acetoacetate
-
692865
Acetobacter aceti
acetoacetyl-CoA + succinate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 55847, ESI-MS, 3 * 55847, calculated, His-tagged recombinant protein
Acetobacter aceti
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acetobacter aceti
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
36.5
-
acetoacetate
-
Acetobacter aceti
36.5
-
acetoacetate
pH 8.0, 25°C
Acetobacter aceti
49
-
acetate
pH 5.0, 25°C
Acetobacter aceti
70.9
-
succinate
-
Acetobacter aceti
70.9
-
succinate
pH 8.0, 25°C
Acetobacter aceti
75.2
-
acetyl-CoA
-
Acetobacter aceti
75.2
-
acetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
147
-
acetate
pH 6.0, 25°C
Acetobacter aceti
201
-
succinyl-CoA
-
Acetobacter aceti
201
-
succinyl-CoA
pH 8.0, 25°C
Acetobacter aceti
280
-
acetate
-
Acetobacter aceti
280
-
acetate
pH 8.0, 25°C
Acetobacter aceti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Acetobacter aceti
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Acetobacter aceti
calculated
-
6.2
General Information
General Information
Commentary
Organism
physiological function
enzyme AarC is succinyl-coenzyme A:acetate CoA-transferase, which replaces succinyl-CoA synthetase in a variant citric acid cycle. This bypass appears to reduce metabolic demand for free CoA, reliance upon nucleotide pools, and the likely effect of variable cytoplasmic pH upon citric acid cycle flux
Acetobacter aceti
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme AarC is succinyl-coenzyme A:acetate CoA-transferase, which replaces succinyl-CoA synthetase in a variant citric acid cycle. This bypass appears to reduce metabolic demand for free CoA, reliance upon nucleotide pools, and the likely effect of variable cytoplasmic pH upon citric acid cycle flux
Acetobacter aceti
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.28
-
acetoacetate
pH 8.0, 25°C
Acetobacter aceti
4
-
acetate
pH 8.0, 25°C
Acetobacter aceti
79
-
succinate
pH 8.0, 25°C
Acetobacter aceti
3400
-
acetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
9000
-
succinyl-CoA
pH 8.0, 25°C
Acetobacter aceti
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.28
-
acetoacetate
pH 8.0, 25°C
Acetobacter aceti
4
-
acetate
pH 8.0, 25°C
Acetobacter aceti
79
-
succinate
pH 8.0, 25°C
Acetobacter aceti
3400
-
acetyl-CoA
pH 8.0, 25°C
Acetobacter aceti
9000
-
succinyl-CoA
pH 8.0, 25°C
Acetobacter aceti
Other publictions for EC 2.8.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738287
Murphy
Functional dissection of the b ...
Acetobacter aceti 1023, Acetobacter aceti
Front. Chem.
4
23
2016
-
-
1
1
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721675
Mullins
Crystal structures of Acetobac ...
Acetobacter aceti 1023, Acetobacter aceti
Biochemistry
51
8422-8434
2012
-
-
1
1
7
-
3
1
-
-
-
1
-
4
-
-
-
1
-
-
-
-
20
-
1
-
-
-
1
-
2
-
3
-
-
-
-
1
-
1
7
-
-
3
3
1
-
-
-
1
-
-
-
-
-
-
-
-
20
-
1
-
-
-
1
-
2
-
-
3
3
-
25
25
692865
Mullins
A specialized citric acid cycl ...
Acetobacter aceti
J. Bacteriol.
190
4933-4940
2008
-
-
1
-
-
-
3
8
-
-
2
-
-
2
-
-
1
1
-
-
4
-
6
1
1
-
-
7
1
-
-
-
5
1
-
-
-
2
-
-
-
-
-
5
8
12
-
-
2
-
-
-
-
1
-
-
4
-
6
1
1
-
-
12
1
-
-
1
-
1
1
-
5
5
693049
van Grinsven
Acetate:succinate CoA-transfer ...
Trichomonas vaginalis
J. Biol. Chem.
283
1411-1418
2008
-
-
1
-
-
-
-
1
1
-
1
-
-
6
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662286
Riviere
Acetyl:succinate CoA-transfera ...
Trypanosoma brucei
J. Biol. Chem.
279
45337-45346
2004
-
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723135
Steinbüchel
Anaerobic pyruvate metabolism ...
Tritrichomonas foetus, Tritrichomonas foetus ATCC 30924
Mol. Biochem. Parasitol.
20
57-65
1986
-
-
-
-
-
-
-
1
1
-
-
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2
-
-
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-
-
-
2
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-