BRENDA - Enzyme Database show
show all sequences of 2.8.3.18

Acetyl:succinate CoA-transferase in procyclic Trypanosoma brucei. Gene identification and role in carbohydrate metabolism

Riviere, L.; van Weelden, S.W.; Glass, P.; Vegh, P.; Coustou, V.; Biran, M.; van Hellemond, J.J.; Bringaud, F.; Tielens, A.G.; Boshart, M.; J. Biol. Chem. 279, 45337-45346 (2004)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
the enzyme seems to be a logical target for broad-spectrum anti-parasitic drugs
Trypanosoma brucei
Cloned(Commentary)
Commentary
Organism
overexpression in Trypanosoma brucei
Trypanosoma brucei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Trypanosoma brucei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate + acetyl-CoA
Trypanosoma brucei
acetate producing pathway
acetate + succinyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
procyclic form
procyclic cell
Trypanosoma brucei
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
knock out mutant
Trypanosoma brucei
0.058
-
wildtype
Trypanosoma brucei
0.157
-
rescued clone, i.e. knock out mutant transfected with the vector pTSArib.ASCT, resulting in overexpression
Trypanosoma brucei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + acetyl-CoA
-
662286
Trypanosoma brucei
acetate + succinyl-CoA
-
-
-
?
succinate + acetyl-CoA
acetate producing pathway
662286
Trypanosoma brucei
acetate + succinyl-CoA
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
medicine
the enzyme seems to be a logical target for broad-spectrum anti-parasitic drugs
Trypanosoma brucei
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Trypanosoma brucei
Trypanosoma brucei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Trypanosoma brucei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate + acetyl-CoA
Trypanosoma brucei
acetate producing pathway
acetate + succinyl-CoA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
procyclic form
procyclic cell
Trypanosoma brucei
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
knock out mutant
Trypanosoma brucei
0.058
-
wildtype
Trypanosoma brucei
0.157
-
rescued clone, i.e. knock out mutant transfected with the vector pTSArib.ASCT, resulting in overexpression
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + acetyl-CoA
-
662286
Trypanosoma brucei
acetate + succinyl-CoA
-
-
-
?
succinate + acetyl-CoA
acetate producing pathway
662286
Trypanosoma brucei
acetate + succinyl-CoA
-
-
-
?
Other publictions for EC 2.8.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738287
Murphy
Functional dissection of the b ...
Acetobacter aceti 1023, Acetobacter aceti
Front. Chem.
4
23
2016
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2
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721675
Mullins
Crystal structures of Acetobac ...
Acetobacter aceti 1023, Acetobacter aceti
Biochemistry
51
8422-8434
2012
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1
1
7
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3
1
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1
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4
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1
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20
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1
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1
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3
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1
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1
7
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3
3
1
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1
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20
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1
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1
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2
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3
3
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25
25
692865
Mullins
A specialized citric acid cycl ...
Acetobacter aceti
J. Bacteriol.
190
4933-4940
2008
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1
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3
8
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2
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2
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1
1
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4
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6
1
1
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7
1
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5
1
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2
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5
8
12
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2
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1
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4
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6
1
1
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12
1
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1
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1
1
-
5
5
693049
van Grinsven
Acetate:succinate CoA-transfer ...
Trichomonas vaginalis
J. Biol. Chem.
283
1411-1418
2008
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1
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1
1
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1
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6
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1
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662286
Riviere
Acetyl:succinate CoA-transfera ...
Trypanosoma brucei
J. Biol. Chem.
279
45337-45346
2004
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1
1
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1
1
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2
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1
3
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723135
Steinbüchel
Anaerobic pyruvate metabolism ...
Tritrichomonas foetus, Tritrichomonas foetus ATCC 30924
Mol. Biochem. Parasitol.
20
57-65
1986
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