Crystallization (Comment) | Organism |
---|---|
crystallization and structure determination of the enzyme/oxalyl-CoA complex, hanging drop technique. Crystallization and structure determination of the D169A, D169E, and D169S mutants | Oxalobacter formigenes |
Protein Variants | Comment | Organism |
---|---|---|
D169A | mutant enzyme is correctly folded and forms interlocked dimers, 1300fold decrease in activity | Oxalobacter formigenes |
D169E | mutant enzyme is correctly folded and forms interlocked dimers, inactive mutant enzyme | Oxalobacter formigenes |
D169S | mutant enzyme is correctly folded and forms interlocked dimers, inactive mutant enzyme | Oxalobacter formigenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
formate | - |
Oxalobacter formigenes | |
oxalyl-CoA | - |
Oxalobacter formigenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
gel filtration | Oxalobacter formigenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oxalobacter formigenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme recombinantly expressed in Escherichia coli | Oxalobacter formigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-CoA + oxalate | involvement of the Asp169 side chain in mediating enzyme-catalyzed CoA transfer via a series of anhydride intermediates | Oxalobacter formigenes | formate + oxalyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Oxalobacter formigenes |
Synonyms | Comment | Organism |
---|---|---|
FRC | - |
Oxalobacter formigenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
oxalate | - |
Oxalobacter formigenes | |
4.3 | - |
formyl-CoA | - |
Oxalobacter formigenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Kis for formate is 17 mM, Kii for formate is 380 mM, Kis for oxalyl-CoA is 0.15 mM, Kii for oxalyl-CoA is 0.28 mM | Oxalobacter formigenes |