Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, monocistronic operon, overexpression in Escherichia coli, activity is similar to the wild-type enzyme | Oxalobacter formigenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, recombinant enzyme, SDS-PAGE | Oxalobacter formigenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-CoA + oxalate | Oxalobacter formigenes | activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora | formate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oxalobacter formigenes | O06644 | gene frc | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli JM109 | Oxalobacter formigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-CoA + oxalate | - |
Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
formyl-CoA + oxalate | activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora | Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
succinyl-CoA + oxalate | no activity with the recombinant enzyme expressed in Escherichia coli | Oxalobacter formigenes | succinate + oxalyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, recombinant enzyme, SDS-PAGE | Oxalobacter formigenes |