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Literature summary for 2.8.3.16 extracted from
- Purification and characterization of formyl-coenzyme A transferase from Oxalobacter formigenes (1990), J. Bacteriol., 172, 3537-3540.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | 20% inhibition at 1 mM | Oxalobacter formigenes |  |
| p-chloromercuribenzoate | 91% inhibition at 1 mM, causes precipitation | Oxalobacter formigenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
succinate | pH 6.7, 25°C | Oxalobacter formigenes | |
| 3 | - |
formyl-CoA | pH 6.7, 25°C | Oxalobacter formigenes | |
| 5.1 | - |
oxalate | pH 6.7, 25°C | Oxalobacter formigenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
1 * 44000, SDS-PAGE | Oxalobacter formigenes |
| 44700 | - |
gel filtration | Oxalobacter formigenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-CoA + oxalate | Oxalobacter formigenes | - |
formate + oxalyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oxalobacter formigenes | O06644 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 31.2fold, to homogeneity | Oxalobacter formigenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.15 | - |
purified enzyme | Oxalobacter formigenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-CoA + oxalate | - |
Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
| additional information | no activity with acetate or malonate | Oxalobacter formigenes | ? | - |
? | |
| succinyl-CoA + oxalate | 11% of the reaction rate observed with formyl-CoA | Oxalobacter formigenes | succinate + oxalyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Oxalobacter formigenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oxalobacter formigenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Oxalobacter formigenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oxalobacter formigenes | - |
4.8 | 4.7 |
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Release 2023.1 (January 2023)
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