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show all sequences of 2.8.3.13

Further purification and characterization of the succinyl-CoA:3-hydroxy-3-methylglutarate coenzyme A transferase from rat-liver mitochondria

Francesconi, M.A.; Donella-Deana, A.; Furlanetto, V.; Cavallini, L.; Palatini, P.; Deana, R.; Biochim. Biophys. Acta 999, 163-170 (1989)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
not activated by 2-mercaptoethanol
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
-
Rattus norvegicus
acetoacetate
-
Rattus norvegicus
acetoacetyl-CoA
non-competitive inhibition
Rattus norvegicus
acetyl-CoA
non-competitive inhibition
Rattus norvegicus
butyryl-CoA
non-competitive inhibition
Rattus norvegicus
carnitine
-
Rattus norvegicus
Cl-
high concentration
Rattus norvegicus
ClO4-
high concentration
Rattus norvegicus
coenzyme A
non-competitive inhibition
Rattus norvegicus
F-
high concentration
Rattus norvegicus
I-
high concentration
Rattus norvegicus
additional information
only negligibly affected by sulfhydryl reagents and by the cations Li+, Cd2+, Ca2+, Mg2+, Mn2+ and Fe2+
Rattus norvegicus
succinate
competitive inhibition of the product
Rattus norvegicus
ZnCl2
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
succinyl-CoA
pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
0.32
-
3-hydroxy-3-methylglutaryl-CoA
pH 7.8, 30°C, cosubstrate succinate or malonate, reverse reaction
Rattus norvegicus
0.73
-
succinate
pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction
Rattus norvegicus
1.78
-
3-hydroxy-3-methylglutarate
pH 7.8, 30°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
52000
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
involved in regulation of the tricarboxylic acid cycle rate and ketogenesis
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.248
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, 0.1 M KH2PO4, pH 7.8, retains 70% of its activity after 2 months of storage
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643833
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643833
Rattus norvegicus
r
succinyl-CoA + 3-hydroxy-3-methylglutarate
involved in regulation of the tricarboxylic acid cycle rate and ketogenesis
643833
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643833
Rattus norvegicus
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 12000-14000, SDS-PAGE
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
not activated by 2-mercaptoethanol
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
-
Rattus norvegicus
acetoacetate
-
Rattus norvegicus
acetoacetyl-CoA
non-competitive inhibition
Rattus norvegicus
acetyl-CoA
non-competitive inhibition
Rattus norvegicus
butyryl-CoA
non-competitive inhibition
Rattus norvegicus
carnitine
-
Rattus norvegicus
Cl-
high concentration
Rattus norvegicus
ClO4-
high concentration
Rattus norvegicus
coenzyme A
non-competitive inhibition
Rattus norvegicus
F-
high concentration
Rattus norvegicus
I-
high concentration
Rattus norvegicus
additional information
only negligibly affected by sulfhydryl reagents and by the cations Li+, Cd2+, Ca2+, Mg2+, Mn2+ and Fe2+
Rattus norvegicus
succinate
competitive inhibition of the product
Rattus norvegicus
ZnCl2
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
succinyl-CoA
pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
0.32
-
3-hydroxy-3-methylglutaryl-CoA
pH 7.8, 30°C, cosubstrate succinate or malonate, reverse reaction
Rattus norvegicus
0.73
-
succinate
pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction
Rattus norvegicus
1.78
-
3-hydroxy-3-methylglutarate
pH 7.8, 30°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
52000
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
involved in regulation of the tricarboxylic acid cycle rate and ketogenesis
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.248
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 0.1 M KH2PO4, pH 7.8, retains 70% of its activity after 2 months of storage
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643833
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643833
Rattus norvegicus
r
succinyl-CoA + 3-hydroxy-3-methylglutarate
involved in regulation of the tricarboxylic acid cycle rate and ketogenesis
643833
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643833
Rattus norvegicus
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 12000-14000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Rattus norvegicus
Other publictions for EC 2.8.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738808
Marlaire
C7orf10 encodes succinate-hydr ...
Homo sapiens
J. Inherit. Metab. Dis.
37
13-19
2014
-
-
1
-
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643834
Deana
Substrate specificity of a dic ...
Rattus norvegicus
Biochem. Int.
26
767-773
1992
-
-
-
-
-
-
-
56
1
-
-
1
-
1
-
-
1
-
-
1
-
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
56
1
-
-
1
-
-
-
1
-
1
-
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643833
Francesconi
Further purification and chara ...
Rattus norvegicus
Biochim. Biophys. Acta
999
163-170
1989
1
-
-
-
-
-
14
4
1
-
1
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
14
-
4
1
-
1
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643832
Deana
Submitochondrial localization ...
Rattus norvegicus
Biochim. Biophys. Acta
662
119-124
1981
1
-
-
-
-
2
3
3
1
-
1
1
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
2
-
3
3
3
1
-
1
1
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-