BRENDA - Enzyme Database show
show all sequences of 2.8.3.13

Submitochondrial localization and partial purification of the succinylCoA: 3-hydroxy-3-methylglutarate coenzyme A transferase from rat liver

Deana, R.; Rigoni, F.; Donella Deana, A.; Galzigna, L.; Biochim. Biophys. Acta 662, 119-124 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
increases activity
Rattus norvegicus
General Stability
General Stability
Organism
addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes
Rattus norvegicus
mercaptoethanol improves stability
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetoacetate
non-competitive inhibition
Rattus norvegicus
malonate
competitive inhibition
Rattus norvegicus
succinate
competitive inhibition of the product
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
succinyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
0.37
-
malonyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
1.7
-
3-hydroxy-3-methylglutarate
pH 8.5, 30°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
mainly in the mitochondrial matrix, also in the inner membrane
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
-
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Wistar albino
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0194
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butyryl-CoA + 3-hydroxy-3-methylglutarate
only negligible amounts of HMGCoA produced
643832
Rattus norvegicus
butyrate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
?
malonyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
malonate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
decreasing the pH to lower values causes an irreversible loss of the enzyme activity
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
acetoacetate
pH 8.5, 30°C
Rattus norvegicus
1.5
-
succinate
pH 8.5, 30°C
Rattus norvegicus
2.1
-
malonate
pH 8.5, 30°C
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
increases activity
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes
Rattus norvegicus
mercaptoethanol improves stability
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetoacetate
non-competitive inhibition
Rattus norvegicus
malonate
competitive inhibition
Rattus norvegicus
succinate
competitive inhibition of the product
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
acetoacetate
pH 8.5, 30°C
Rattus norvegicus
1.5
-
succinate
pH 8.5, 30°C
Rattus norvegicus
2.1
-
malonate
pH 8.5, 30°C
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
succinyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
0.37
-
malonyl-CoA
pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate
Rattus norvegicus
1.7
-
3-hydroxy-3-methylglutarate
pH 8.5, 30°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
mainly in the mitochondrial matrix, also in the inner membrane
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + 3-hydroxy-3-methylglutarate
Rattus norvegicus
-
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
Rattus norvegicus
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0194
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butyryl-CoA + 3-hydroxy-3-methylglutarate
only negligible amounts of HMGCoA produced
643832
Rattus norvegicus
butyrate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
?
malonyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
malonate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
succinyl-CoA + 3-hydroxy-3-methylglutarate
-
643832
Rattus norvegicus
succinate + 3-hydroxy-3-methylglutaryl-CoA
-
643832
Rattus norvegicus
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
decreasing the pH to lower values causes an irreversible loss of the enzyme activity
Rattus norvegicus
Other publictions for EC 2.8.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738808
Marlaire
C7orf10 encodes succinate-hydr ...
Homo sapiens
J. Inherit. Metab. Dis.
37
13-19
2014
-
-
1
-
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643834
Deana
Substrate specificity of a dic ...
Rattus norvegicus
Biochem. Int.
26
767-773
1992
-
-
-
-
-
-
-
56
1
-
-
1
-
1
-
-
1
-
-
1
-
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
56
1
-
-
1
-
-
-
1
-
1
-
-
32
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643833
Francesconi
Further purification and chara ...
Rattus norvegicus
Biochim. Biophys. Acta
999
163-170
1989
1
-
-
-
-
-
14
4
1
-
1
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
14
-
4
1
-
1
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643832
Deana
Submitochondrial localization ...
Rattus norvegicus
Biochim. Biophys. Acta
662
119-124
1981
1
-
-
-
-
2
3
3
1
-
1
1
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
2
-
3
3
3
1
-
1
1
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-