BRENDA - Enzyme Database show
show all sequences of 2.8.3.11

Efficient conversion of itaconic acid to (S)-(+)-citramalic acid by Alcaligenes xylosoxydans IL 142

He, B.F.; Ozawa, T.; Nakajima-Kambe, T.; Nakahara, T.; J. Biosci. Bioeng. 89, 388-391 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
activity is enhanced in presence of CoA and succinate
Achromobacter xylosoxidans
CoA
activity is enhanced in presence of ATP and succinate
Achromobacter xylosoxidans
succinate
activity is enhanced in presence of CoA and ATP
Achromobacter xylosoxidans
Application
Application
Commentary
Organism
biotechnology
production process of enantiomeric pure (S)-(+)-citramalic acid from itaconic acid
Achromobacter xylosoxidans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate + (S)-(+)-citramalyl-CoA
Achromobacter xylosoxidans
99.9% enantiomeric purity
succinyl-CoA + citramalate
-
Achromobacter xylosoxidans
r
succinate + (S)-(+)-citramalyl-CoA
Achromobacter xylosoxidans IL142
99.9% enantiomeric purity
succinyl-CoA + citramalate
-
Achromobacter xylosoxidans IL142
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Achromobacter xylosoxidans
-
-
-
Achromobacter xylosoxidans IL142
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + (3S)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans
r
succinate + (3S)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans IL142
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans IL142
r
succinate + (S)-(+)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans
r
succinate + (S)-(+)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans IL142
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans IL142
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Achromobacter xylosoxidans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Achromobacter xylosoxidans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
activity is enhanced in presence of CoA and succinate
Achromobacter xylosoxidans
CoA
activity is enhanced in presence of ATP and succinate
Achromobacter xylosoxidans
succinate
activity is enhanced in presence of CoA and ATP
Achromobacter xylosoxidans
Application (protein specific)
Application
Commentary
Organism
biotechnology
production process of enantiomeric pure (S)-(+)-citramalic acid from itaconic acid
Achromobacter xylosoxidans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate + (S)-(+)-citramalyl-CoA
Achromobacter xylosoxidans
99.9% enantiomeric purity
succinyl-CoA + citramalate
-
Achromobacter xylosoxidans
r
succinate + (S)-(+)-citramalyl-CoA
Achromobacter xylosoxidans IL142
99.9% enantiomeric purity
succinyl-CoA + citramalate
-
Achromobacter xylosoxidans IL142
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate + (3S)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans
r
succinate + (3S)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans IL142
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans IL142
r
succinate + (S)-(+)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans
r
succinate + (S)-(+)-citramalyl-CoA
99.9% enantiomeric purity
645951
Achromobacter xylosoxidans IL142
succinyl-CoA + citramalate
-
645951
Achromobacter xylosoxidans IL142
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Achromobacter xylosoxidans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Achromobacter xylosoxidans
Other publictions for EC 2.8.3.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645951
He
Efficient conversion of itacon ...
Achromobacter xylosoxidans, Achromobacter xylosoxidans IL142
J. Biosci. Bioeng.
89
388-391
2000
3
1
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2
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3
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4
-
1
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1
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3
1
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-
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-
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2
-
-
-
-
-
-
-
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4
-
1
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-
1
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-
-
-
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-
33256
Dimroth
Isolation and function of the ...
Clostridium tetanomorphum
Eur. J. Biochem.
80
469-477
1977
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1
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1
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5
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1
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1
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1
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1
1
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1
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5
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1
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1
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