BRENDA - Enzyme Database show
show all sequences of 2.8.3.1

Engineering Propionibacterium freudenreichii subsp. shermanii for enhanced propionic acid fermentation: effects of overexpressing propionyl-CoA:succinate CoA transferase

Wang, Z.; Ammar, E.M.; Zhang, A.; Wang, L.; Lin, M.; Yang, S.T.; Metab. Eng. 27, 46-56 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Propionibacterium freudenreichii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55700
-
-
Propionibacterium freudenreichii
56000
-
x * 56000, SDS-PAGE
Propionibacterium freudenreichii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
propionyl-CoA + succinate
Propionibacterium freudenreichii
-
propanoate + succinoyl-CoA
-
-
?
propionyl-CoA + succinate
Propionibacterium freudenreichii DSM 4902
-
propanoate + succinoyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Propionibacterium freudenreichii
-
Propionibacterium freudenreichii subsp. shermanii
-
Propionibacterium freudenreichii DSM 4902
-
Propionibacterium freudenreichii subsp. shermanii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
propionyl-CoA + succinate
-
738980
Propionibacterium freudenreichii
propanoate + succinoyl-CoA
-
-
-
?
propionyl-CoA + succinate
-
738980
Propionibacterium freudenreichii DSM 4902
propanoate + succinoyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55700, calculated from amino acid sequence; x * 56000, SDS-PAGE
Propionibacterium freudenreichii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Propionibacterium freudenreichii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55700
-
-
Propionibacterium freudenreichii
56000
-
x * 56000, SDS-PAGE
Propionibacterium freudenreichii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
propionyl-CoA + succinate
Propionibacterium freudenreichii
-
propanoate + succinoyl-CoA
-
-
?
propionyl-CoA + succinate
Propionibacterium freudenreichii DSM 4902
-
propanoate + succinoyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
propionyl-CoA + succinate
-
738980
Propionibacterium freudenreichii
propanoate + succinoyl-CoA
-
-
-
?
propionyl-CoA + succinate
-
738980
Propionibacterium freudenreichii DSM 4902
propanoate + succinoyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55700, calculated from amino acid sequence; x * 56000, SDS-PAGE
Propionibacterium freudenreichii
Other publictions for EC 2.8.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739448
Luo
Production of 3-hydroxypropion ...
Megasphaera elsdenii
PLoS ONE
11
e0156286
2016
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1
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738980
Wang
Engineering Propionibacterium ...
Propionibacterium freudenreichii, Propionibacterium freudenreichii DSM 4902
Metab. Eng.
27
46-56
2015
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1
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1
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737535
Volodina
Characterization of propionate ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
98
3579-3589
2014
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1
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1
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4
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2
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41
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1
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32
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32
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725884
Li
Feedstocks affect the diversit ...
uncultured bacterium
Microb. Ecol.
66
351-362
2013
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5
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736535
Park
Propionyl-CoA dependent biosyn ...
Anaerotignum propionicum
J. Biotechnol.
165
93-98
2013
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1
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1
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6
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2
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724088
Lindenkamp
A propionate CoA-transferase o ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
97
7699-7709
2012
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1
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3
2
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43
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1
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15
1
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1
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2
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1
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15
1
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725223
Yang
Biosynthesis of poly(3-hydroxy ...
Cupriavidus necator
J. Appl. Microbiol.
113
815-823
2012
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1
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2
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1
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724059
Han
Chemo-enzymatic synthesis of p ...
Anaerotignum propionicum, Anaerotignum propionicum JCM 1430
Appl. Microbiol. Biotechnol.
92
509-517
2011
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2
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2
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10
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10
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702792
Yang
Biosynthesis of polylactic aci ...
Anaerotignum propionicum
Biotechnol. Bioeng.
105
150-160
2010
-
1
1
-
1
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1
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5
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1
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1
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702793
Jung
Metabolic engineering of Esche ...
Anaerotignum propionicum
Biotechnol. Bioeng.
105
161-171
2010
-
1
1
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1
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3
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694994
Sode
Propionate sensor using coenzy ...
Anaerotignum propionicum
Protein Pept. Lett.
15
779-781
2008
1
2
1
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4
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672769
Rajashekhara
Volatile fatty acid-sensing sy ...
Anaerotignum propionicum
Biotechnol. Prog.
22
334-337
2006
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2
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1
1
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661640
Brock
On the mechanism of action of ...
Aspergillus nidulans
Eur. J. Biochem.
271
3227-3241
2004
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645949
Selmer
Propionate CoA-transferase fro ...
Anaerotignum propionicum
Eur. J. Biochem.
269
372-380
2002
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7
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1
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33928
Schweiger
On the dehydration of (R)-lact ...
Anaerotignum propionicum
FEBS Lett.
171
79-84
1984
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5
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1
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1
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645947
Stadtman
-
Acyl-coenzyme A synthesis by p ...
Clostridium kluyveri
Fed. Proc.
11
291
1952
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