BRENDA - Enzyme Database show
show all sequences of 2.8.3.1

A propionate CoA-transferase of Ralstonia eutropha H16 with broad substrate specificity catalyzing the CoA thioester formation of various carboxylic acids

Lindenkamp, N.; Schuermann, M.; Steinbuechel, A.; Appl. Microbiol. Biotechnol. 97, 7699-7709 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
4 * 57000, calculated from amino acid sequence
Cupriavidus necator
64000
-
4 * 64000, SDS-PAGE
Cupriavidus necator
228000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetate + propanoyl-CoA
Cupriavidus necator
-
acetyl-CoA + propanoate
-
-
?
acetate + propanoyl-CoA
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
acetyl-CoA + propanoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Purification (Commentary)
Commentary
Organism
Ni Sepharose column chromatography
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxybutyrate + acetyl-CoA
75% activity compared to propionate
724088
Cupriavidus necator
acetate + 3-hydroxybutanoyl-CoA
-
-
-
?
3-hydroxybutyrate + acetyl-CoA
75% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 3-hydroxybutanoyl-CoA
-
-
-
?
3-hydroxypropanoate + acetyl-CoA
80% activity compared to propionate
724088
Cupriavidus necator
acetate + 3-hydroxypropanoyl-CoA
-
-
-
?
3-hydroxypropanoate + acetyl-CoA
80% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 3-hydroxypropanoyl-CoA
-
-
-
?
4-hydroxybutyrate + acetyl-CoA
19% activity compared to propionate
724088
Cupriavidus necator
acetate + 4-hydroxybutanoyl-CoA
-
-
-
?
4-hydroxybutyrate + acetyl-CoA
19% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 4-hydroxybutanoyl-CoA
-
-
-
?
acetate + propanoyl-CoA
-
724088
Cupriavidus necator
acetyl-CoA + propanoate
-
-
-
?
acetate + propanoyl-CoA
-
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetyl-CoA + propanoate
-
-
-
?
acrylate + acrylyl-CoA
69% activity compared to propionate
724088
Cupriavidus necator
acetate + propionyl-CoA
-
-
-
?
butyrate + acetyl-CoA
28% activity compared to propionate
724088
Cupriavidus necator
acetate + butyryl-CoA
-
-
-
?
crotonate + acetyl-CoA
28% activity compared to propionate
724088
Cupriavidus necator
acetate + crotonyl-CoA
-
-
-
?
lactate + acetyl-CoA
36% activity compared to propionate
724088
Cupriavidus necator
acetate + lactoyl-CoA
-
-
-
?
propionate + acetyl-CoA
propionate is the best substrate, 100% activity
724088
Cupriavidus necator
acetate + propionyl-CoA
-
-
-
?
succinate + acetyl-CoA
48% activity compared to propionate
724088
Cupriavidus necator
acetate + succinyl-CoA
-
-
-
?
succinate + acetyl-CoA
48% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + succinyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 57000, calculated from amino acid sequence; 4 * 64000, SDS-PAGE
Cupriavidus necator
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
4 * 57000, calculated from amino acid sequence
Cupriavidus necator
64000
-
4 * 64000, SDS-PAGE
Cupriavidus necator
228000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetate + propanoyl-CoA
Cupriavidus necator
-
acetyl-CoA + propanoate
-
-
?
acetate + propanoyl-CoA
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
acetyl-CoA + propanoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni Sepharose column chromatography
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxybutyrate + acetyl-CoA
75% activity compared to propionate
724088
Cupriavidus necator
acetate + 3-hydroxybutanoyl-CoA
-
-
-
?
3-hydroxybutyrate + acetyl-CoA
75% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 3-hydroxybutanoyl-CoA
-
-
-
?
3-hydroxypropanoate + acetyl-CoA
80% activity compared to propionate
724088
Cupriavidus necator
acetate + 3-hydroxypropanoyl-CoA
-
-
-
?
3-hydroxypropanoate + acetyl-CoA
80% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 3-hydroxypropanoyl-CoA
-
-
-
?
4-hydroxybutyrate + acetyl-CoA
19% activity compared to propionate
724088
Cupriavidus necator
acetate + 4-hydroxybutanoyl-CoA
-
-
-
?
4-hydroxybutyrate + acetyl-CoA
19% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + 4-hydroxybutanoyl-CoA
-
-
-
?
acetate + propanoyl-CoA
-
724088
Cupriavidus necator
acetyl-CoA + propanoate
-
-
-
?
acetate + propanoyl-CoA
-
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetyl-CoA + propanoate
-
-
-
?
acrylate + acrylyl-CoA
69% activity compared to propionate
724088
Cupriavidus necator
acetate + propionyl-CoA
-
-
-
?
butyrate + acetyl-CoA
28% activity compared to propionate
724088
Cupriavidus necator
acetate + butyryl-CoA
-
-
-
?
crotonate + acetyl-CoA
28% activity compared to propionate
724088
Cupriavidus necator
acetate + crotonyl-CoA
-
-
-
?
lactate + acetyl-CoA
36% activity compared to propionate
724088
Cupriavidus necator
acetate + lactoyl-CoA
-
-
-
?
propionate + acetyl-CoA
propionate is the best substrate, 100% activity
724088
Cupriavidus necator
acetate + propionyl-CoA
-
-
-
?
succinate + acetyl-CoA
48% activity compared to propionate
724088
Cupriavidus necator
acetate + succinyl-CoA
-
-
-
?
succinate + acetyl-CoA
48% activity compared to propionate
724088
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
acetate + succinyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 57000, calculated from amino acid sequence; 4 * 64000, SDS-PAGE
Cupriavidus necator
Other publictions for EC 2.8.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739448
Luo
Production of 3-hydroxypropion ...
Megasphaera elsdenii
PLoS ONE
11
e0156286
2016
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1
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1
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5
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1
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1
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1
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1
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-
-
-
738980
Wang
Engineering Propionibacterium ...
Propionibacterium freudenreichii, Propionibacterium freudenreichii DSM 4902
Metab. Eng.
27
46-56
2015
-
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1
-
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2
2
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4
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2
1
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1
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2
2
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2
1
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737535
Volodina
Characterization of propionate ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
98
3579-3589
2014
-
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1
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1
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4
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2
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41
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1
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32
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4
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1
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32
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725884
Li
Feedstocks affect the diversit ...
uncultured bacterium
Microb. Ecol.
66
351-362
2013
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5
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736535
Park
Propionyl-CoA dependent biosyn ...
Anaerotignum propionicum
J. Biotechnol.
165
93-98
2013
-
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1
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1
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6
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2
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724088
Lindenkamp
A propionate CoA-transferase o ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Appl. Microbiol. Biotechnol.
97
7699-7709
2012
-
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1
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-
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-
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3
2
-
43
-
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1
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-
-
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15
1
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1
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3
2
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1
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15
1
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-
725223
Yang
Biosynthesis of poly(3-hydroxy ...
Cupriavidus necator
J. Appl. Microbiol.
113
815-823
2012
-
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1
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2
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1
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724059
Han
Chemo-enzymatic synthesis of p ...
Anaerotignum propionicum, Anaerotignum propionicum JCM 1430
Appl. Microbiol. Biotechnol.
92
509-517
2011
-
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2
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2
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10
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10
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702792
Yang
Biosynthesis of polylactic aci ...
Anaerotignum propionicum
Biotechnol. Bioeng.
105
150-160
2010
-
1
1
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1
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1
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5
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702793
Jung
Metabolic engineering of Esche ...
Anaerotignum propionicum
Biotechnol. Bioeng.
105
161-171
2010
-
1
1
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1
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694994
Sode
Propionate sensor using coenzy ...
Anaerotignum propionicum
Protein Pept. Lett.
15
779-781
2008
1
2
1
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672769
Rajashekhara
Volatile fatty acid-sensing sy ...
Anaerotignum propionicum
Biotechnol. Prog.
22
334-337
2006
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661640
Brock
On the mechanism of action of ...
Aspergillus nidulans
Eur. J. Biochem.
271
3227-3241
2004
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645949
Selmer
Propionate CoA-transferase fro ...
Anaerotignum propionicum
Eur. J. Biochem.
269
372-380
2002
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33928
Schweiger
On the dehydration of (R)-lact ...
Anaerotignum propionicum
FEBS Lett.
171
79-84
1984
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5
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1
7
1
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5
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1
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645947
Stadtman
-
Acyl-coenzyme A synthesis by p ...
Clostridium kluyveri
Fed. Proc.
11
291
1952
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