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Literature summary for 2.8.3.1 extracted from

  • Rajashekhara, E.; Hosoda, A.; Sode, K.; Ikenaga, H.; Watanabe, K.
    Volatile fatty acid-sensing system involving coenzyme-A transferase (2006), Biotechnol. Prog., 22, 334-337.
    View publication on PubMed
Search for more literature for 2.8.3.1

Application

Application Comment Organism
biotechnology establishing of an on-site monitoring of volatile fatty acids VFA, such as propionate, biosensing system using recombinant propionate CoA transferase and recombinant acyl-CoA oxidase, this system produces hydrogen peroxide in the presence of acetyl-CoA, oxygen, and VFA substrates, which can be quantified by colorimetric methods using peroxidase and dye reagents, e.g., 4-aminobenzoic acid plus 4-aminoantipyrine, overview Anaerotignum propionicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 propionate pH 7.0, 25°C Anaerotignum propionicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + propionate Anaerotignum propionicum
-
 acetate + propanoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Anaerotignum propionicum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of propionate-detection systems, overview Anaerotignum propionicum
0.34
-
 substrate propionate Anaerotignum propionicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + butyrate low activity Anaerotignum propionicum acetate + butanoyl-CoA
-
 ?
acetyl-CoA + propionate
-
 Anaerotignum propionicum acetate + propanoyl-CoA
-
 ?
additional information valerate is no substrate Anaerotignum propionicum ?
-
 ?

Synonyms

Synonyms Comment Organism
PCT
-
 Anaerotignum propionicum
propionate CoA transferase
-
 Anaerotignum propionicum
propionate coenzyme A transferase
-
 Anaerotignum propionicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Anaerotignum propionicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 over 90% of maximal activity within this range Anaerotignum propionicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.2
-
 propionate pH 7.0, 25°C Anaerotignum propionicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Anaerotignum propionicum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 over 90% of maximal activity within this range Anaerotignum propionicum

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Anaerotignum propionicum