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Literature summary for 2.8.2.9 extracted from

  • Sheng, J.J.; Saxena, A.; Duffel, M.W.
    Influence of phenylalanines 77 and 138 on the stereospecificity of aryl sulfotransferase IV (2004), Drug Metab. Dispos., 32, 559-565.
    View publication on PubMed
Search for more literature for 2.8.2.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
F138A 25fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol. Unlike the wild-type enzyme the mutant enzyme is also active with (S)-(+)-1,2,3,4-tetrahydro-1-naphthol Rattus norvegicus
F77A 83.3fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol,unlike the wild-type enzyme the mutant enzyme is also active with (S)-(+)-1,2,3,4-tetrahydro-1-naphthol Rattus norvegicus
Y236A 116.7fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 (R)-(-)-1,2,3,4-tetrahydro-1-naphthol wild-type enzyme Rattus norvegicus
0.3
-
 (R)-(-)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme F138A Rattus norvegicus
0.5
-
 (S)-(+)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme F77A Rattus norvegicus
1
-
 (R)-(-)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme F77A Rattus norvegicus
1.4
-
 (R)-(-)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme Y236A Rattus norvegicus
1.8
-
 (S)-(+)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme F138A Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + (R)-(-)-1,2,3,4-tetrahydro-1-naphthol activity is abserved with wild-type enzyme and mutant enzymes F77A, F138A and Y236A Rattus norvegicus adenosine 3',5'-bisphosphate + (R)-(-)-1,2,3,4-tetrahydro-1-naphthyl sulfate
-
 ?
3'-phosphoadenylylsulfate + (S)-(+)-1,2,3,4-tetrahydro-1-naphthol mutant enzyme F77A and F138A show activity, wild-type enzyme and mutant enzyme Y236A show no activity Rattus norvegicus adenosine 3',5'-bisphosphate + (S)-(+)-1,2,3,4-tetrahydro-1-naphthyl sulfate
-
 ?