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Literature summary for 2.8.2.9 extracted from

  • Saidha, T.; Schiff, J.A.
    Purification and properties of a phenol sulphotransferase from Euglena using L-tyrosine as substrate (1994), Biochem. J., 298, 45-50.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.9

General Stability

General Stability Organism
instable to freezing Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate PAP, competitive to 3'-phosphoadenylylsulfate Euglena gracilis
Tyrosine O4-sulfate uncompetitive to L-tyrosine Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 L-tyrosine pH 7, 37°C Euglena gracilis
0.08
-
 L-tyrosine tert-butyl ester pH 7, 37°C Euglena gracilis
0.1
-
 2-naphthol L-tyrosine ethyl ester, pH 7, 37°C Euglena gracilis
0.133
-
 L-tyrosine benzyl ester pH 7, 37°C Euglena gracilis
0.15
-
 L-tyrosine methyl ester pH 7, 37°C Euglena gracilis
0.2
-
 3-methoxy-4-hydroxyphenylglycol L-tyrosinamide, pH 7, 37°C Euglena gracilis
0.35
-
 AlaTyr pH 7, 37°C Euglena gracilis
0.37
-
 L-tyrosine ethyl ester pH 7, 37°C Euglena gracilis
0.39 0.4 3-chlorophenol pH 7, 37°C Euglena gracilis
0.416
-
 3-Nitrophenol pH 7, 37°C Euglena gracilis
0.5
-
 2-chlorophenol pH 7, 37°C Euglena gracilis
0.55
-
 4-Chlorophenol pH 7, 37°C Euglena gracilis
0.714
-
 L-4-hydroxyphenylglycine pH 7, 37°C Euglena gracilis
0.833
-
 Tyr-Gly pH 7, 37°C Euglena gracilis
1.25
-
 4-nitrophenol pH 7, 37°C Euglena gracilis
2.5
-
 GlyTyr pH 7, 37°C Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
 var. bacillaris, W10BSmL, aplastidic mutant
-

Purification (Commentary)

Purification (Comment) Organism
-
 Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2053
-
-
 Euglena gracilis

Storage Stability

Storage Stability Organism
4°C, 10 mM potassium phosphate, pH 6.8, 250 mM sucrose and 0.02% w/v NaN3, no more than 10% loss of activity after 5 months Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 2-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 2-naphthol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 3-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 3-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + 4-nitrophenol
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + Ala-Tyr
-
 Euglena gracilis adenosine 3',5'-bisphosphate + Ala-(O-sulfo)Tyr
-
 ?
3'-phosphoadenylylsulfate + GlyTyr
-
 Euglena gracilis adenosine 3',5'-bisphosphate + Gly-(O-sulfo)Tyr
-
 ?
3'-phosphoadenylylsulfate + L-4-hydroxyphenylglycine
-
 Euglena gracilis adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine O4-sulfate
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine allyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine allyl ester 4-sulfate
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine amide
-
 Euglena gracilis adenosine 3',5'-bisphosphate + O-sulfo-L-tyrosine amide
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine benzyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine benzyl ester 4-sulfate
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine ethyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine ethyl ester 4-sulfate
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine methyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
-
 ?
3'-phosphoadenylylsulfate + L-tyrosine tert-butyl ester
-
 Euglena gracilis adenosine 3',5'-bisphosphate + tyrosine tert-butyl ester 4-sulfate
-
 ?
3'-phosphoadenylylsulfate + nitrophenol 3-nitrophenol or 4-nitrophenol Euglena gracilis adenosine 3',5'-bisphosphate + nitrophenyl sulfate
-
 ?
3'-phosphoadenylylsulfate + TyrGly
-
 Euglena gracilis adenosine 3',5'-bisphosphate + 4O-sulfo-Tyr-Gly
-
 ?
additional information not: adenosine 5'-phosphosulfate Euglena gracilis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Euglena gracilis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Euglena gracilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 adenosine 3',5'-bisphosphate pH 7, 37°C Euglena gracilis
0.5
-
 Tyrosine O4-sulfate pH 7, 37°C Euglena gracilis