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Literature summary for 2.8.2.8 extracted from

  • Gesteira, T.F.; Coulson-Thomas, V.J.; Taunay-Rodrigues, A.; Oliveira, V.; Thacker, B.E.; Juliano, M.A.; Pasqualini, R.; Arap, W.; Tersariol, I.L.; Nader, H.B.; Esko, J.D.; Pinhal, M.A.
    Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1 (2011), J. Biol. Chem., 286, 5338-5346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in pgsA745 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
CNMQALSMPVTC the peptide inhibits isoform Ndst1 activity by direct interaction with the enzyme near the active site, the peptide inhibits sulfotransferase activity by 25 and 40% at 0.01 and 0.1 mM, respectively Mus musculus
CRGWRGEKIGNC the peptide blocks the binding of substrate to the enzyme isoform Ndst1 Mus musculus
NMQALSMPVT
-
 Mus musculus
RGWRGEKIGN
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 N-desulfoheparin apparent value, in 50 mM HEPES, pH 7.0, at 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine Mus musculus
-
 adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
-
 ?
3'-phosphoadenylylsulfate + N-desulfoheparin Mus musculus
-
 adenosine 3',5'-bisphosphate + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG-Sepharose bead chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine
-
 Mus musculus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
-
 ?
3'-phosphoadenylylsulfate + N-desulfoheparin
-
 Mus musculus adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
N-deacetylase-N-sulfotransferase-1
-
 Mus musculus
Ndst1
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
 NMQALSMPVT apparent value, in 50 mM HEPES, pH 7.0, at 37°C Mus musculus
0.48
-
 RGWRGEKIGN apparent value, in 50 mM HEPES, pH 7.0, at 37°C Mus musculus