Cloned (Comment) | Organism |
---|---|
isozymes NDST1 and NDST2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | lack of NDST1 affects heparan sulfate structure in all tissues tested, with a dramatic reduction in N- and O-sulfation of the polysaccharide. Silencing the expression of NDST1, NDST2, 2-OST, and 3-OST3 by RNA interference efficiently decreases binding and activity of cyclophilin B | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | Homo sapiens | NDST1 is able to partially sulfate exogenous substrate in the absence of NDST2 but not vice versa | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
additional information | Homo sapiens | NDST1 and NDST2 have no redundant activities but rather have complementary activities in making N-sulfated heparan sulfate motifs with cyclophilin B-binding properties. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes NDST1 and NDST2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
JURKAT cell | clone E6-1, ATCC TIB-152 | Homo sapiens | - |
macrophage | high expression level of isozyme NDST2 | Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
monocyte | high expression level of isozyme NDST2. The expression of NDST2 in monocytes is modified following cell activation or maturation, although the same treatment has no significant effect on the level of mRNAencoding NDST1 | Homo sapiens | - |
additional information | NDST1 and NDST2 are widely expressed in all the tissues analyzed, overview. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases | Homo sapiens | - |
T-47D cell | - |
Homo sapiens | - |
T-lymphocyte | CD4+ cells, high expression level of isozyme NDST2. NDST1 is barely detected in naive T-lymphocytes by comparison with memory and activated CD4 T cells, whereas no notable variation in the expression of NDST2 can be distinguished between T cell subsets | Homo sapiens | - |
THP-1 cell | promonocytic leukemia cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | NDST1 is able to partially sulfate exogenous substrate in the absence of NDST2 but not vice versa | Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
additional information | NDST1 and NDST2 have no redundant activities but rather have complementary activities in making N-sulfated heparan sulfate motifs with cyclophilin B-binding properties. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-deacetylase/N-sulfotransferase | - |
Homo sapiens |
Ndst1 | - |
Homo sapiens |
NDST2 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | in the general scheme of heparan sulfate biosynthesis, GlcNAc N-deacetylation and N-sulfation by NDSTs create the prerequisite substrate needed for the next enzymatic modifications | Homo sapiens |
physiological function | NDST1 is required for initiation of N-sulfation of the nascent precursor, whereas NDST2 may fill in or extend the sections of N-sulfated residues in heparin and highly sulfated heparan sulfate species | Homo sapiens |