Literature summary for 2.8.2.8 extracted from

Kakuta, Y.; Sueyoshi, T.; Negishi, M.; Pedersen, L.C.
Crystal structure of the sulfotransferase domain of human heparan sulfate N-deacetylase/N-sulfotransferase 1 (1999), J. Biol. Chem., 274, 10673-10676.

Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the selenomethionyl sulfotransferase domain NST1 in methionine auxotrophic Escherichia coli strain B834 (DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
18 mg/ml, 0.1 M Tris-HCl, pH 8.3, 0.1 M NaCl, 4 mM 3'-phosphoadenosine 5'-phosphate, reservoir solution: Bis-Tris-propane, pH 7.0, 0.2 M NaCl, 30% PEG 3000, room temperature, X-ray diffraction structure determination and analysis	Homo sapiens
purified recombinant selenomethionyl sulfotransferase domain NST1	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	bifunctional enzyme possessing heparan sulfate N-deacetylase and N-sulfotransferase activity	-
Homo sapiens	-	crystal structure of sulfotransferase domain, Protein Data Bank: 1NST	-
Homo sapiens	-	enzyme form HSNST1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant sulfotransferase domain NST1 from Escherichia coli strain B834 (DE3)	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine	5'-phosphate binding and active site structure	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine	-	Homo sapiens	adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine	-	?

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Release 2023.1 (January 2023)