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Literature summary for 2.8.2.8 extracted from

  • Toma, L.; Berninsone, P.; Hirschberg, C.B.
    The putative heparin-specific N-acetylglucosaminyl N-Deacetylase/N-sulfotransferase also occurs in non-heparin-producing cells (1998), J. Biol. Chem., 273, 22458-22465.
    View publication on PubMed
Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment) Organism
lambdagt10 library construction and screening, DNA sequence determination and analysis, expression in COS-1 cells Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 N-deacetylase K5-polysaccharide
-
 Bos taurus
0.0173
-
 3'-phosphoadenylylsulfate
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi vesicle lumen Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions required Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Bos taurus bifunctional enzyme initiates heparin and heparan sulfate biosynthesis adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Bos taurus enzyme is present in embryonic tracheal tissue, which does not synthesize heparin adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus O97583 bifunctional enzyme possessing heparan sulfate N-deacetylase and N-sulfotransferase activity
-

Purification (Commentary)

Purification (Comment) Organism
N-acetylglucosaminyl N-deacetylase/N-sulfotransferase, recombinant from COS-1 cells, 1-step affinity chromatographical purification Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture CCL 44 cell Bos taurus
-
cell culture embryonic trachea Bos taurus
-
EBTr (NBL-4) cell
-
 Bos taurus
-
embryo trachea, cell culture Bos taurus
-
additional information tissue-specific heparin-synthesizing enzyme forms Bos taurus
-
trachea embryonic, cell culture Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in both reaction directions Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + N-deacetylase K5-polysaccharide
-
 Bos taurus ?
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
-
 Bos taurus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 r
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine bifunctional enzyme initiates heparin and heparan sulfate biosynthesis Bos taurus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine enzyme is present in embryonic tracheal tissue, which does not synthesize heparin Bos taurus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylylsulfate + heparin
-
 Bos taurus adenosine 3',5'-bisphosphate + N-sulfoheparin
-
 ?
3'-phosphoadenylylsulfate + N-deacetylated K5-polysaccharide
-
 Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
heparin/heparan sulfate N-deacetylase/N-sulfotransferase
-
 Bos taurus
N-acetylglucosaminyl N-deacetylase/N-sulfotransferase
-
 Bos taurus