Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
3'-phosphoadenylylsulfate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi vesicle | membrane | Rattus norvegicus | - |
- |
membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 22% of the activity with Mn2+ | Rattus norvegicus | |
Mg2+ | metal ion required, maximal activity with 5 mM | Rattus norvegicus | |
Mn2+ | 62% of the activity with Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
1 * 94000, SDS-PAGE | Rattus norvegicus |
97000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | Rattus norvegicus | the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.95 | - |
substrate heparan sulfate | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 6 months in presence of 20% glycerol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | substrate needs to be deacetylated | Rattus norvegicus | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains | Rattus norvegicus | adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine | - |
? | |
3'-phosphoadenylylsulfate + heparan sulfate | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + N-sulfoheparan sulfate | - |
? | |
3'-phosphoadenylylsulfate + heparin | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + N-sulfoheparin | - |
? | |
3'-phosphoadenylylsulfate + N-desulfated heparin | best substrate | Rattus norvegicus | ? | - |
? | |
additional information | no acceptors: tyrosine-containing tripeptides, N-acetylated heparan sulfate, N-acetylated heparin, chondroitin | Rattus norvegicus | ? | - |
? | |
additional information | chondroitin sulfate is no acceptor | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 94000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8.1 | pH 5.7: 33% of activity maximum, pH 8.1: 54% of activity maximum | Rattus norvegicus |