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Literature summary for 2.8.2.8 extracted from

  • Brandan, E.; Hirschberg, C.B.
    Purification of rat liver N-heparan-sulfate sulfotransferase (1988), J. Biol. Chem., 263, 2417-2422.
    View publication on PubMed
Search for more literature for 2.8.2.8

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 3'-phosphoadenylylsulfate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi vesicle membrane Rattus norvegicus
-
-
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 22% of the activity with Mn2+ Rattus norvegicus
Mg2+ metal ion required, maximal activity with 5 mM Rattus norvegicus
Mn2+ 62% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 1 * 94000, SDS-PAGE Rattus norvegicus
97000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Rattus norvegicus the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.95
-
 substrate heparan sulfate Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, stable for at least 6 months in presence of 20% glycerol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine substrate needs to be deacetylated Rattus norvegicus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains Rattus norvegicus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylylsulfate + heparan sulfate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + N-sulfoheparan sulfate
-
 ?
3'-phosphoadenylylsulfate + heparin
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + N-sulfoheparin
-
 ?
3'-phosphoadenylylsulfate + N-desulfated heparin best substrate Rattus norvegicus ?
-
 ?
additional information no acceptors: tyrosine-containing tripeptides, N-acetylated heparan sulfate, N-acetylated heparin, chondroitin Rattus norvegicus ?
-
 ?
additional information chondroitin sulfate is no acceptor Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 94000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8.1 pH 5.7: 33% of activity maximum, pH 8.1: 54% of activity maximum Rattus norvegicus