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Literature summary for 2.8.2.8 extracted from

  • Wei, Z.; Swiedler, S.J.; Ishihara, M.; Orellana, A.; Hirschberg, C.B.
    A single protein catalyzes both N-deacetylation and N-sulfation during the biosynthesis of heparan sulfate (1993), Proc. Natl. Acad. Sci. USA, 90, 3885-3888.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.8

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the bifunctional enzyme in CHO cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3',5'-ADP
-
 Rattus norvegicus
additional information NEM: no effect Rattus norvegicus
NaCl above 200 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi vesicle lumen Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine Rattus norvegicus N-sulfated residues of heparan sulfate participate in the binding of this polymer to proteins as basic fibroblast growth factor adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 a single protein possesses both N-deacetylase and N-sulfotransferase activity
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine substrate needs to be deacetylated Rattus norvegicus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine N-sulfated residues of heparan sulfate participate in the binding of this polymer to proteins as basic fibroblast growth factor Rattus norvegicus adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
 ?
3'-phosphoadenylylsulfate + heparan sulfate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + N-sulfoheparan sulfate
-
 ?