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Literature summary for 2.8.2.8 extracted from
- Purification and characterization of 3-phosphoadenylylsulfate: N-desulfoheparan sulfate sulfotransferase from arterial tissue (1984), Eur. J. Biochem., 138, 301-308.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.89 | - |
N-desulfoheparan sulfate | calculated from disaccharide units | Bos taurus | |
| 2.5 | - |
N,O-desulfoheparan sulfate tetrasaccharide | calculated from disaccharide units | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 41% of the activity with Mn2+ | Bos taurus |  |
| Mg2+ | 52% of the activity with Mn2+ | Bos taurus | |
| Mn2+ | divalent cation required | Bos taurus | |
| Mn2+ | maximal activation at: 5 mM | Bos taurus | |
| Mn2+ | Mn2+ most effective | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
peaks with enzyme activity: 200000 and 110000 Da | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-desulfo-N-acetylheparan sulfate deacetylase activity copurifies | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| artery | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + heparin | - |
Bos taurus | adenosine 3',5'-bisphosphate + N-sulfoheparin | - |
? | |
| 3'-phosphoadenylylsulfate + N,O-desulfated heparan sulfate | - |
Bos taurus | adenosine 3',5'-bisphosphate + O-desulfated heparan sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + N,O-desulfoheparan sulfate tetrasaccharide | - |
Bos taurus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + N,O-desulfoheparan sulfate tetrasaccharides with the nonreducing terminus occupied by glucuronic acid | not iduronic acid | Bos taurus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + N,O-desulfoheparan sulfate tetrasaccharides with the nonreducing terminus occupied by glucuronic acid | the enzyme transfers sulfate to the 2-amino groups and to the 6-hydroxy groups of glucosamine units of the acceptor substrate, the ratio of the N/O-sulfation ranges between 3:1 and 2:1 | Bos taurus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + N-desulfoheparan sulfate | - |
Bos taurus | adenosine 3',5'-bisphosphate + heparan sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + oligosaccharides derived from N-desulfoheparan sulfate | - |
Bos taurus | ? | - |
? | |
| additional information | poor acceptors: N-desulfo-N-acetylheparan, heparin, N-desulfoheparin | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Bos taurus |
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