Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | - |
Aspergillus nidulans | |
glycol | - |
Aspergillus nidulans | |
n-Propanol | maximal activation, at 2.5 mM, inhibition above 25 mM | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Naphthylamine | - |
Aspergillus nidulans | |
2,4-dinitrophenol | - |
Aspergillus nidulans | |
3-Aminophenol | - |
Aspergillus nidulans | |
4-Dimethylaminophenol | - |
Aspergillus nidulans | |
4-nitrophenol | competitive inhibition | Aspergillus nidulans | |
Acetylcholine | - |
Aspergillus nidulans | |
beta-homocholine | - |
Aspergillus nidulans | |
carnitine | competitive inhibition | Aspergillus nidulans | |
Co2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
cyanide | - |
Aspergillus nidulans | |
diethanolamine | - |
Aspergillus nidulans | |
Dimethylaminoethanethiol | - |
Aspergillus nidulans | |
dimethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
Dimethylaminopropan-1-ol | competitive inhibition | Aspergillus nidulans | |
Dimethylaminopropen-1-ol | - |
Aspergillus nidulans | |
dimethylethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
ethyltrimethylammonium bromide | - |
Aspergillus nidulans | |
Fe3+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
gamma-homocholine | - |
Aspergillus nidulans | |
hexadecyltrimethylammonium bromide | - |
Aspergillus nidulans | |
Mn2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
additional information | iodoacetate is less effective as inhibitor, di-isopropylfluorophosphate is without action | Aspergillus nidulans | |
N-ethylmaleimide | - |
Aspergillus nidulans | |
n-Propanol | concentrations above 0.025 M | Aspergillus nidulans | |
Ni2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
p-chloromercuribenzoate | - |
Aspergillus nidulans | |
phenol | strong inhibitor | Aspergillus nidulans | |
Prostigmine | - |
Aspergillus nidulans | |
sulfate | - |
Aspergillus nidulans | |
thiocholine | - |
Aspergillus nidulans | |
triethanolamine | - |
Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0222 | - |
3'-phosphoadenylylsulfate | pH 7.3, 37°C | Aspergillus nidulans | |
12 | - |
choline | pH 7.3, 37°C | Aspergillus nidulans | |
20 | - |
N,N-dimethylethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans | |
25 | - |
dimethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not even a partial requirement for Mg2+ | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + choline | Aspergillus nidulans | - |
adenosine 3',5'-bisphosphate + choline sulfate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
CMI 38,595 S eta, sulfateless mutant | - |
Purification (Comment) | Organism |
---|---|
partial | Aspergillus nidulans |
Storage Stability | Organism |
---|---|
-20°C, freezing and storing for 3 days results in complete loss of activity | Aspergillus nidulans |
2°C, more stable after (NH4)2SO4 or acetone fractionation, activity is reduced by 85% during 4 weeks | Aspergillus nidulans |
2°C, very unstable, loses 95% of its activity after 5 days | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + choline | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
3'-phosphoadenylylsulfate + choline | sulfate transfer appears to be irreversible in vitro | Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate | - |
? | |
3'-phosphoadenylylsulfate + N,N-dimethylethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylethylaminoethanol O-sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
no loss of activity during 10 min at pH 9.2, 30% loss at pH 7.3, 34% loss at pH 5.0 | Aspergillus nidulans |
55 | - |
almost completely inactivated | Aspergillus nidulans |
58.5 | - |
complete loss of activity at pH 5.0, 85% activity retained at pH 9.2 | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | about 50% of activity maximum at pH 5.8 and 10.1 | Aspergillus nidulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 12 | stable at room temperature in the pH range 8.0-11.0, above pH 11.0 enzyme is rapidly inactivated, 80% inactivation at pH 12.0, below pH 8.0 stablity declines slowly with decreasing pH, maximum stability at pH 8,5-10.5 | Aspergillus nidulans |