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Literature summary for 2.8.2.4 extracted from
- The transition of human estrogen sulfotransferase from generalist to specialist using directed enzyme evolution (2012), J. Mol. Biol., 416, 21-32.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D65N/K85E/E86K/F138Y | the mutant exhibits increased thermostability (heat inactivation at 43.4°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K | the mutant exhibits increased thermostability (heat inactivation at 43.1°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| E12G/N29D/F138Y/G182S/L251V | the mutant exhibits increased thermostability (heat inactivation at 51.8°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| E12G/N29D/V73A/N87D/F138Y/G182S/L195M/E211G/E285G | the mutant exhibits increased thermostability (heat inactivation at 52.1°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| F138Y/G182S/H224K/E285G | the mutant exhibits increased thermostability (heat inactivation at 54.6°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| F138Y/G182S/L195M/E211G | the mutant exhibits increased thermostability (heat inactivation at 55.8°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| K85E/A135T/F138Y/G182S/L251V | the mutant exhibits increased thermostability (heat inactivation at 46.3°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| N29D/L195M/H224K/L251V | the mutant exhibits increased thermostability (heat inactivation at 48.3°C) compared to the wild type enzyme (42°C) | Homo sapiens |
| V73A/N87D/G182S/H224K/L251V/E285G | the mutant exhibits increased thermostability (heat inactivation at 52.8°C) compared to the wild type enzyme (42°C) | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + estrone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P49888 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography, and gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + 17beta-estradiol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl sulfate + 3-cyano-7-hydroxycoumarin | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 3-cyano-7-hydroxycoumaryl sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 4-nitrophenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + estrone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| estrogen sulfotransferase | - |
Homo sapiens |
| SULT1E1 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
the wild type enzyme is inactivated at 42°C | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
3-cyano-7-hydroxycoumarin | mutant enzyme K85E/A135T/F138Y/G182S/L251V, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.0009 | - |
4-nitrophenol | mutant enzyme K85E/A135T/F138Y/G182S/L251V, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.01 | - |
4-nitrophenol | mutant enzyme E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.059 | - |
4-nitrophenol | mutant enzyme D65N/K85E/E86K/F138Y, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.065 | - |
3-cyano-7-hydroxycoumarin | mutant enzyme E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.151 | - |
4-nitrophenol | wild type enzyme, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.179 | - |
3-cyano-7-hydroxycoumarin | mutant enzyme D65N/K85E/E86K/F138Y, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
| 0.694 | - |
3-cyano-7-hydroxycoumarin | wild type enzyme, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication | Homo sapiens | |
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