Cloned (Comment) | Organism |
---|---|
EST cloned and expressed in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C84F | site-directed mutagenesis | Mus musculus |
C84Y | site-directed mutagenesis | Mus musculus |
F142A | site-directed mutagenesis | Mus musculus |
F142L | site-directed mutagenesis | Mus musculus |
F24A | site-directed mutagenesis | Mus musculus |
I146A | site-directed mutagenesis | Mus musculus |
M247G | site-directed mutagenesis | Mus musculus |
N86A | site-directed mutagenesis | Mus musculus |
R23A | site-directed mutagenesis | Mus musculus |
S148A | site-directed mutagenesis | Mus musculus |
Y149F | site-directed mutagenesis | Mus musculus |
Y240F | site-directed mutagenesis | Mus musculus |
Y81A | site-directed mutagenesis | Mus musculus |
Y81F | site-directed mutagenesis | Mus musculus |
Y81L | site-directed mutagenesis | Mus musculus |
Y81L/F142L | site-directed mutagenesis | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
estradiol | pH 8.0, 37°C wild-type | Mus musculus | |
0.000032 | - |
estradiol | pH 8.0, 37°C mutant Y81F | Mus musculus | |
0.000092 | - |
estradiol | pH 8.0, 37°C mutant Y81L | Mus musculus | |
0.000235 | - |
estradiol | pH 8.0, 37°C mutant Y81A | Mus musculus | |
0.000735 | - |
estradiol | pH 8.0, 37°C mutant Y81L/F142L | Mus musculus | |
0.000812 | - |
estradiol | pH 8.0, 37°C mutant F142L | Mus musculus | |
0.000868 | - |
androstenediol | pH 8.0, 37°C mutant Y81L | Mus musculus | |
0.00125 | - |
estradiol | pH 8.0, 37°C mutant F142A | Mus musculus | |
0.0029 | - |
androstenediol | pH 8.0, 37°C mutant F142L | Mus musculus | |
0.003224 | - |
androstenediol | pH 8.0, 37°C wild-type | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + estradiol | Mus musculus | - |
adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + androstenediol | wild-type EST exhibits little ability to sulfate AD | Mus musculus | adenosine 3',5'-bisphosphate + androstenediol sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Mus musculus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estradiol | - |
Mus musculus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r |