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Literature summary for 2.8.2.4 extracted from

  • Petrotchenko, E.V.; Doerflein, M.E.; Kakuta, Y.; Pedersen, L.C.; Negishi, M.
    Substrate gating confers steroid specificity to estrogen sulfotransferase (1999), J. Biol. Chem., 274, 30019-30022.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
EST cloned and expressed in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
C84F site-directed mutagenesis Mus musculus
C84Y site-directed mutagenesis Mus musculus
F142A site-directed mutagenesis Mus musculus
F142L site-directed mutagenesis Mus musculus
F24A site-directed mutagenesis Mus musculus
I146A site-directed mutagenesis Mus musculus
M247G site-directed mutagenesis Mus musculus
N86A site-directed mutagenesis Mus musculus
R23A site-directed mutagenesis Mus musculus
S148A site-directed mutagenesis Mus musculus
Y149F site-directed mutagenesis Mus musculus
Y240F site-directed mutagenesis Mus musculus
Y81A site-directed mutagenesis Mus musculus
Y81F site-directed mutagenesis Mus musculus
Y81L site-directed mutagenesis Mus musculus
Y81L/F142L site-directed mutagenesis Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000025
-
estradiol pH 8.0, 37°C wild-type Mus musculus
0.000032
-
estradiol pH 8.0, 37°C mutant Y81F Mus musculus
0.000092
-
estradiol pH 8.0, 37°C mutant Y81L Mus musculus
0.000235
-
estradiol pH 8.0, 37°C mutant Y81A Mus musculus
0.000735
-
estradiol pH 8.0, 37°C mutant Y81L/F142L Mus musculus
0.000812
-
estradiol pH 8.0, 37°C mutant F142L Mus musculus
0.000868
-
androstenediol pH 8.0, 37°C mutant Y81L Mus musculus
0.00125
-
estradiol pH 8.0, 37°C mutant F142A Mus musculus
0.0029
-
androstenediol pH 8.0, 37°C mutant F142L Mus musculus
0.003224
-
androstenediol pH 8.0, 37°C wild-type Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + estradiol Mus musculus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + androstenediol wild-type EST exhibits little ability to sulfate AD Mus musculus adenosine 3',5'-bisphosphate + androstenediol sulfate
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Mus musculus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
3'-phosphoadenylylsulfate + estradiol
-
Mus musculus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r