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Literature summary for 2.8.2.4 extracted from

  • Tomizuka, T.; Oeda, T.; Tamura, Y.; Yoshida, S.; Strott, C.A.
    Characterization of guinea pig estrogen sulfotransferase expressed by Chinese hamster ovary cell-K1 stable transfectants (1994), Endocrinology, 135, 938-943.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
EST cloned and expressed by CHO-K1 cells stably transfected with plasmid pcDNAI/Neo eukaryotic expression vector Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenylylsulfate competitive inhibition Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
estriol pH 7.4, 37°C Cavia porcellus
0.00006
-
estrone pH 7.4, 37°C Cavia porcellus
0.00007
-
17beta-estradiol pH 7.4, 37°C Cavia porcellus
0.0012
-
3'-phosphoadenylylsulfate pH 7.4, 37°C Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cavia porcellus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
native and recombinant enzyme, immunoblot analysis Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
guinea pig
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + estriol
-
Cavia porcellus adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
?
3'-phosphoadenylylsulfate + estrone
-
Cavia porcellus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00052
-
3'-phosphoadenylylsulfate pH 7.4, 37°C, competitive inhibition Cavia porcellus

pI Value

Organism Comment pI Value Maximum pI Value
Cavia porcellus isoelectric focusing, multiple charge isoforms
-
5.2
Cavia porcellus isoelectric focusing, multiple charge isoforms
-
5.4
Cavia porcellus isoelectric focusing, multiple charge isoforms
-
5.5
Cavia porcellus isoelectric focusing, multiple charge isoforms
-
6.4