Cloned (Comment) | Organism |
---|---|
EST cloned and expressed by CHO-K1 cells stably transfected with plasmid pcDNAI/Neo eukaryotic expression vector | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-phosphoadenylylsulfate | competitive inhibition | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
estriol | pH 7.4, 37°C | Cavia porcellus | |
0.00006 | - |
estrone | pH 7.4, 37°C | Cavia porcellus | |
0.00007 | - |
17beta-estradiol | pH 7.4, 37°C | Cavia porcellus | |
0.0012 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cavia porcellus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
native and recombinant enzyme, immunoblot analysis | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
guinea pig | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + estriol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estriol 3-sulfate | - |
? | |
3'-phosphoadenylylsulfate + estrone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
3'-phosphoadenylylsulfate | pH 7.4, 37°C, competitive inhibition | Cavia porcellus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cavia porcellus | isoelectric focusing, multiple charge isoforms | - |
5.2 |
Cavia porcellus | isoelectric focusing, multiple charge isoforms | - |
5.4 |
Cavia porcellus | isoelectric focusing, multiple charge isoforms | - |
5.5 |
Cavia porcellus | isoelectric focusing, multiple charge isoforms | - |
6.4 |