Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cavia porcellus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + estradiol | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estradiol | Cavia porcellus Hartley | - |
adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | Cavia porcellus Hartley | - |
adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
guinea pig | - |
Cavia porcellus | - |
english shorthair | - |
Cavia porcellus | - |
Hartley | - |
Cavia porcellus Hartley | - |
Hartley | - |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Cavia porcellus | - |
ovary | - |
Cavia porcellus | - |
testis | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0274 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
-20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months | Cavia porcellus |
-20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity | Cavia porcellus |
0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + estradiol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estradiol | - |
Cavia porcellus Hartley | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | - |
Cavia porcellus Hartley | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000-36000, SDS-PAGE | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cavia porcellus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cavia porcellus | 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1 | - |
5.5 |
Cavia porcellus | 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1 | - |
6.1 |