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Literature summary for 2.8.2.4 extracted from

  • Hobkirk, R.; Glasier, M.A.; Brown, L.Y.
    Purification and some characteristics of an oestrogen sulphotransferase from guinea pig adrenal gland and its non-identity with adrenal pregnenolone sulphotransferase (1990), Biochem. J., 268, 759-764.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cavia porcellus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + estradiol Cavia porcellus
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r
3'-phosphoadenylylsulfate + estradiol Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r
3'-phosphoadenylylsulfate + estrone Cavia porcellus
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
r
3'-phosphoadenylylsulfate + estrone Cavia porcellus Hartley
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
r

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
guinea pig
-
Cavia porcellus
-
english shorthair
-
Cavia porcellus
-
Hartley
-
Cavia porcellus Hartley
-
Hartley
-

Purification (Commentary)

Purification (Comment) Organism
-
Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Cavia porcellus
-
ovary
-
Cavia porcellus
-
testis
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0274
-
-
Cavia porcellus

Storage Stability

Storage Stability Organism
-20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months Cavia porcellus
-20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity Cavia porcellus
0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + estradiol
-
Cavia porcellus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r
3'-phosphoadenylylsulfate + estradiol
-
Cavia porcellus Hartley adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
r
3'-phosphoadenylylsulfate + estrone
-
Cavia porcellus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
r
3'-phosphoadenylylsulfate + estrone
-
Cavia porcellus Hartley adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 34000-36000, SDS-PAGE Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Cavia porcellus

pI Value

Organism Comment pI Value Maximum pI Value
Cavia porcellus 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
5.5
Cavia porcellus 2 forms of the enzyme, FPLC chromatofocusing in a pH gradient of 4.0-7.1
-
6.1