Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | non-competitive inhibition | Bos taurus | |
Co2+ | strong inhibition | Bos taurus | |
additional information | only moderately sensitive to SH-blocking agents, no inhibition with EDTA up to 20 mM | Bos taurus | |
Ni2+ | strong inhibition | Bos taurus | |
o-Iodosobenzoate | sensitive above 10 mM | Bos taurus | |
p-chloromercuribenzoate | sensitive above 1 mM | Bos taurus | |
Zn2+ | strong inhibition | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
17beta-estradiol | pH 8.1, 37°C | Bos taurus | |
0.07 | - |
adenosine 3',5'-bisphosphate | pH 8.1, 37°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activated by, but no absolute requirement | Bos taurus | |
Mg2+ | activated by, but no absolute requirement | Bos taurus | |
Mn2+ | activated by, but no absolute requirement | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + estrone | Bos taurus | steroid metabolism | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
0°C, partially purified A form of enzyme loses only a small percentage of its activity on standing in ice for a period of 2 weeks, B form loses 50% of its initial activity in this time | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 17beta-estradiol | - |
Bos taurus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estriol | - |
Bos taurus | adenosine 3',5'-bisphosphate + estriol 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | - |
Bos taurus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + estrone | steroid metabolism | Bos taurus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + hexestrol | - |
Bos taurus | adenosine 3',5'-bisphosphate + hexestrol 4-sulfate | - |
r | |
3'-phosphoadenylylsulfate + stilbestrol | - |
Bos taurus | adenosine 3',5'-bisphosphate + stilbestrol 4-sulfate | - |
r | |
additional information | simple phenols, 2-naphthylamine and 3beta-hydroxysteroids are not sulfated, dienestrol is no substrate, dehydroepiandrosterone, phenol, p-nitrophenol, alpha-naphthol, beta-naphthol, beta-naphthylamine and 17 beta-estradiol-3-methyl ether fail to act as substrates | Bos taurus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
ADP | pH 8.1, 37°C | Bos taurus |