Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2.5 to 3.5fold activation | squid | |
Ca2+ | 2.5 to 3.5fold activation | squid | |
Co2+ | 2.5 to 3.5fold activation | squid | |
KCl | 2fold activation | squid | |
Mg2+ | 2.5 to 3.5fold activation | squid | |
Mn2+ | 2.5 to 3.5fold activation | squid | |
NaCl | 2fold activation | squid | |
protamine | 2fold activation | squid | |
Sr2+ | 2.5 to 3.5fold activation | squid |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
3'-phosphoadenylyl sulfate | pH 6.8, 25°C | squid | |
0.0011 | - |
chondroitin sulfate A | pH 6.8, 25°C | squid | |
0.0013 | - |
dermatan sulfate | pH 6.8, 25°C | squid |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
1 * 63000, SDS-PAGE | squid |
66000 | - |
gel filtration | squid |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
squid | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains N-linked oligosaccharides | squid |
Purification (Comment) | Organism |
---|---|
- |
squid |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | - |
squid | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 4-sulfated chondroitin pentasaccharide | best substrate, sulfation only with nonreducing terminal GalNAc(4-SO4) residues | squid | adenosine 3',5'-bisphosphate + 4,6'-sulfated chondroitin pentasaccharide | - |
? | |
3'-phosphoadenylyl sulfate + 4-sulfated chondroitin trisaccharide | 95.2% of activity compared with 4-sulfated pentasaccharide | squid | adenosine 3',5'-bisphosphate + 4,6'-sulfated chondroitin trisaccharide | - |
? | |
3'-phosphoadenylyl sulfate + chondroitin sulfate A | - |
squid | adenosine 3',5'-bisphosphate + chondroitin sulfate A 6'-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + chondroitin sulfate C | - |
squid | adenosine 3',5'-bisphosphate + chondroitin sulfate C 6'-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + dermatan sulfate | - |
squid | adenosine 3',5'-bisphosphate + 6'-sulfated dermatan sulfate | - |
? | |
additional information | sulfatation occurs mainly at position 6 of the internal N-acetylgalactosamine 4-sulfate residue | squid | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE | squid |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
squid |