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Literature summary for 2.8.2.32 extracted from

  • Macrides, T.A.; Faktor, D.A.; Kalafatis, N.; Amiet, R.G.
    Enzymic sulfation of bile salts. Partial purification and characterization of an enzyme from the liver of the shark Heterodontus portusjacksoni that catalyses the sulfation of the shark bile steroid 5 beta-scymnol (1994), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 107, 461-469.
    View publication on PubMed
Search for more literature for 2.8.2.32

Inhibitors

Inhibitors Comment Organism Structure
adenosine-3',5'-diphosphate 0.1 mM, 98% inhibition Heterodontus portusjacksoni
iodoacetate 0.1 mM, 99% inhibition Heterodontus portusjacksoni
NaN3 0.1 mM, 65% inhibition Heterodontus portusjacksoni
p-chloromercuribenzoate 0.1 mM, 67% inhibition Heterodontus portusjacksoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 3'-phosphoadenosine-5'-phosphosulfate pH 6.5 Heterodontus portusjacksoni
0.014
-
 5beta-scymnol pH 6.5 Heterodontus portusjacksoni

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Heterodontus portusjacksoni 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Heterodontus portusjacksoni
40000
-
 native PAGE, probably two isoenzymes Heterodontus portusjacksoni
45000
-
 native PAGE, probably two isoenzymes Heterodontus portusjacksoni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol Heterodontus portusjacksoni
-
 adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Heterodontus portusjacksoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydroxylapatite, Sephadex G-100 Heterodontus portusjacksoni

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Heterodontus portusjacksoni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000000275
-
-
 Heterodontus portusjacksoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol
-
 Heterodontus portusjacksoni adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?
3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol sulfation occurs at either the C-26 or C-27 position Heterodontus portusjacksoni adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE, probably two isoenzymes Heterodontus portusjacksoni
monomer 1 * 45000, SDS-PAGE, probably two isoenzymes Heterodontus portusjacksoni

pI Value

Organism Comment pI Value Maximum pI Value
Heterodontus portusjacksoni isoelectric focusing, probably two isoenzymes
-
 5
Heterodontus portusjacksoni isoelectric focusing, probably two isoenzymes
-
 5.85