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Literature summary for 2.8.2.32 extracted from

  • Pettigrew, N.E.; Wright, P.F.A.; Macrides, T.A.
    5beta-Scymnol sulfotransferase isolated from the tissues of an Australian shark species (1998), Comp. Biochem. Physiol. B, 121B, 299-307.
No PubMed abstract available

General Stability

General Stability Organism
freeze-thawing reduces activity, enzyme has very low stability at 4°C especially during concentration procedures Heterodontus portusjacksoni

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-diphosphate competitive inhibition Heterodontus portusjacksoni
EDTA 50 mM, 89% inhibition Heterodontus portusjacksoni
iodoacetic acid 0.1 mM, 29% inhibition Heterodontus portusjacksoni
NaN3 0.1 mM, 39% inhibition Heterodontus portusjacksoni
p-chloromercuribenzoate 0.1 mM, 38% inhibition Heterodontus portusjacksoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
(24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol room temperature, pH 6.5 Heterodontus portusjacksoni
0.0044
-
3'-phosphoadenosine 5'-phosphosulfate room temperature, pH 6.5 Heterodontus portusjacksoni

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Heterodontus portusjacksoni 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol Heterodontus portusjacksoni trivial name 5beta-scymnol adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?

Organism

Organism UniProt Comment Textmining
Heterodontus portusjacksoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydroxyapatite, DEAE-Sephacel, Sephadex G-100, partially purified Heterodontus portusjacksoni

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Heterodontus portusjacksoni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000002
-
-
Heterodontus portusjacksoni

Storage Stability

Storage Stability Organism
-80°C, at least 12 months, no loss of activity Heterodontus portusjacksoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol trivial name 5beta-scymnol Heterodontus portusjacksoni adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?
3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol
-
Heterodontus portusjacksoni adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate
-
?
3'-phosphoadenosine 5'-phosphosulfate + testosterone
-
Heterodontus portusjacksoni adenosine 3',5'-diphosphate + testosterone sulfate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Heterodontus portusjacksoni

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45
-
Heterodontus portusjacksoni

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00037
-
adenosine 3',5'-diphosphate vs. 3'-phosphoadenosine 5'-phosphosulfate Heterodontus portusjacksoni
0.00084
-
adenosine 3',5'-diphosphate vs. 5beta-scymnol Heterodontus portusjacksoni