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Literature summary for 2.8.2.28 extracted from

  • Varin, L.; Ibrahim, R.K.
    Partial Purification and some properties of flavonol 7-sulfotransferase from flaveria bidentis (1991), Plant Physiol., 95, 1254-1258.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.28

Inhibitors

Inhibitors Comment Organism Structure
Flavonol substrate below 0.0015 mM, inhibition Flaveria bidentis
additional information EDTA, SH-group reagents at 1-10 mM, no inhibition Flaveria bidentis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 quercetin 3,3'-disulfate isoenzyme II Flaveria bidentis
0.00024
-
 quercetin 3,3'-disulfate isoenzyme I Flaveria bidentis
0.00033
-
 3'-phosphoadenylylsulfate cosubstrate quercetin 3,3'-disulfate, isoenzyme I Flaveria bidentis
0.00046
-
 3'-phosphoadenylylsulfate cosubstrate quercetin 3,3'-disulfate, isoenzyme II Flaveria bidentis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for a divalent cation at 1-10 mM Flaveria bidentis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Flaveria bidentis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + quercetin 3,3'-bissulfate Flaveria bidentis isoenzyme I and II quercetin 3,3',7-trissulfate + adenosine 3',5'-bisphosphate
-
 ?
additional information Flaveria bidentis involved in biosynthesis of polysulfated flavonols in Flaveria bidentis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Flaveria bidentis
-
-
-
Flaveria chlorifolia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Flaveria bidentis

Source Tissue

Source Tissue Comment Organism Textmining
shoot tip
-
 Flaveria bidentis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00038
-
 isoenzyme II Flaveria bidentis
0.00058
-
 isoenzyme I Flaveria bidentis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate isoenzyme I and II Flaveria bidentis isorhamnetin 3,7-disulfate + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenylylsulfate + quercetin 3,3'-bissulfate isoenzyme I and II Flaveria bidentis quercetin 3,3',7-trissulfate + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenylylsulfate + quercetin 3,4'-bissulfate isoenzyme I and II Flaveria bidentis quercetin 3,4',7-trissulfate + adenosine 3',5'-bisphosphate
-
 ?
additional information quercetin, quercetin 3-sulfate, quercetin 3'-sulfate, flavones (apigenin, luteolin), phenylpropanoids (p-coumaric, caffeic or ferulic acids) are no substrates Flaveria bidentis ?
-
 ?
additional information involved in biosynthesis of polysulfated flavonols in Flaveria bidentis Flaveria bidentis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 phosphate buffer Flaveria bidentis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 about 40-45% of maximal activity at pH 6.5 and 8.5 Flaveria bidentis

pI Value

Organism Comment pI Value Maximum pI Value
Flaveria bidentis chromatofocusing, isoform II 6.8 6.3
Flaveria bidentis chromatofocusing, isoform I
-
 6.5