Any feedback?
Literature summary for 2.8.2.25 extracted from
- Novel flavonol 3-sulfotransferase. Purification, kinetic properties, and partial amino acid sequence (1992), J. Biol. Chem., 267, 1858-1863.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3',5'-diphosphoadenosine | competitive with respect to 3'-phosphoadenylylsulfate, noncompetitive with respect to quercetin | Flaveria chlorifolia |  |
| additional information | EDTA, SH-reagents at 1 and 10 mM, e.g. p-chloromercuribenzoate, iodoacetate or iodoacetamide, are not inhibitory | Flaveria chlorifolia | |
| quercetin 3-sulfate | noncompetitive with respect to quercetin or 3'-phosphoadenylylsulfate | Flaveria chlorifolia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00018 | - |
3'-phosphoadenylylsulfate | cosubstrate quercetin, pH 6.0 | Flaveria chlorifolia | |
| 0.0002 | - |
quercetin | pH 6.0 | Flaveria chlorifolia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no divalent cation required | Flaveria chlorifolia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
1 * 34500, SDS-PAGE | Flaveria chlorifolia |
| 35000 | - |
gel filtration | Flaveria chlorifolia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + quercetin | Flaveria chlorifolia | - |
adenosine 3',5'-bisphosphate + quercetin 3-sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flaveria chlorifolia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Flaveria chlorifolia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plant | - |
Flaveria chlorifolia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + isorhamnetin | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + kaempferol | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + kaempferol 3-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + patuletin | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + patuletin 3-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + quercetin | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + quercetin 3-sulfate | - |
? | |
| 3'-phosphoadenylylsulfate + rhamnetin | - |
Flaveria chlorifolia | adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate | - |
? | |
| additional information | quercetagetin, gossypetin, myricetin or galangin are no substrates | Flaveria chlorifolia | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34500, SDS-PAGE | Flaveria chlorifolia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Flaveria chlorifolia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
and pH 8.5, two maxima | Flaveria chlorifolia |
| 8.5 | - |
and pH 6, two maxima | Flaveria chlorifolia |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
