Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)cells | Desulfitobacterium hafniense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | in acetone the activity of the enzyme decreases to 30-34% of the original activity | Desulfitobacterium hafniense | |
dimethylformamide | in dimethylformamide the activity of the enzyme decreases to 30-34% of the original activity | Desulfitobacterium hafniense | |
dioxane | in dioxane the activity of the enzyme decreases to 30-34% of the original activity | Desulfitobacterium hafniense | |
tetrahydrofuran | in tetrhydrofuran the activity of the enzyme decreases to 30-34% of the original activity | Desulfitobacterium hafniense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Desulfitobacterium hafniense |
71400 | - |
x * 71400, calculated from amino acid sequence | Desulfitobacterium hafniense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + phenol | Desulfitobacterium hafniense | 100% activity | 4-nitrophenol + phenyl sulfate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | the enzyme is stable in the presence of 10% (v/v) of DMSO and even in 25% of DMSO, the remaining activity is still 733 | Desulfitobacterium hafniense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfitobacterium hafniense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Poros column chromatography and Q-FF column chromatography | Desulfitobacterium hafniense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + 1-butanol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + butyl sulfate | - |
? | |
4-nitrophenyl sulfate + 1-naphthol | 50% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 1-naphththyl sulfate | - |
? | |
4-nitrophenyl sulfate + 1-octanol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + octyl sulfate | - |
? | |
4-nitrophenyl sulfate + 1-pentanol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + pentyl sulfate | - |
? | |
4-nitrophenyl sulfate + 1-phenylethanol | 2-10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + 1-phenylethyl sulfate | - |
? | |
4-nitrophenyl sulfate + 17beta-estradiol | after 24 h about 80% of the 17beta-estradiol is converted | Desulfitobacterium hafniense | 4-nitrophenol + ? | - |
? | |
4-nitrophenyl sulfate + 2-aminophenol | 136% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 2-aminophenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 2-butanol | 2-10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + 2-butyl sulfate | - |
? | |
4-nitrophenyl sulfate + 2-naphthol | 86% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 2-naphthyl sulfate | - |
? | |
4-nitrophenyl sulfate + 2-octanol | 2-10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + 2-octyl sulfate | - |
? | |
4-nitrophenyl sulfate + 2-phenylethanol | 50% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + 2-phenylethyl sulfate | - |
? | |
4-nitrophenyl sulfate + 3,5-dimethoxyphenol | 33% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 3,5-dimethoxyphenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 3-chlorophenol | 138% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 3-chlorophenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 4-aminophenol | 102% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 4-aminophenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 4-chlorophenol | 195% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 4-chlorophenyl sulfate | - |
? | |
4-nitrophenyl sulfate + 4-cresol | 200% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 4-cresol sulfate | - |
? | |
4-nitrophenyl sulfate + 4-hydroxybenzoic acid | 0.7% activity compared to phenol | Desulfitobacterium hafniense | ? | - |
? | |
4-nitrophenyl sulfate + 4-hydroxyindole | 88% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + indol-4-yl sulfate | - |
? | |
4-nitrophenyl sulfate + 5-hydroxyindole | 74% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + indol-5-yl sulfate | - |
? | |
4-nitrophenyl sulfate + benzyl alcohol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + benzyl sulfate | - |
? | |
4-nitrophenyl sulfate + cyclohexanol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + cyclohexyl sulfate | - |
? | |
4-nitrophenyl sulfate + D-glucose | 2-10% conversion in 7 days | Desulfitobacterium hafniense | ? | - |
? | |
4-nitrophenyl sulfate + dihydroxyacetone | 2-10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + ? | - |
? | |
4-nitrophenyl sulfate + dopamine | 540% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + ? | - |
? | |
4-nitrophenyl sulfate + estrone | after 24 h 90% of the estrone is sulfated | Desulfitobacterium hafniense | 4-nitrophenol + estrone 3-sulfate | - |
? | |
4-nitrophenyl sulfate + furfuryl alcohol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + furfuryl sulfate | - |
? | |
4-nitrophenyl sulfate + glycerol | 10% conversion in 7 days | Desulfitobacterium hafniense | 4-nitrophenol + ? | - |
? | |
4-nitrophenyl sulfate + Leu-enkephalin | within 48 h 91% of Leu-enkephalin is sulfated which reaches 98% after 6 days of incubation | Desulfitobacterium hafniense | 4-nitrophenol + Leu-enkephalin sulfate | - |
? | |
4-nitrophenyl sulfate + N-hydroxysuccinimide | 2-10% conversion in 7 days | Desulfitobacterium hafniense | ? | - |
? | |
4-nitrophenyl sulfate + octyl glucoside | 2-10% conversion in 7 days | Desulfitobacterium hafniense | ? | - |
? | |
4-nitrophenyl sulfate + phenol | 100% activity | Desulfitobacterium hafniense | 4-nitrophenol + phenyl sulfate | - |
? | |
4-nitrophenyl sulfate + resorcinol | 70% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + resorcinyl sulfate | - |
? | |
4-nitrophenyl sulfate + tyramine | 60% activity compared to phenol | Desulfitobacterium hafniense | 4-nitrophenol + 4-(2-aminoethyl)phenyl sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Desulfitobacterium hafniense |
? | x * 71400, calculated from amino acid sequence | Desulfitobacterium hafniense |
Synonyms | Comment | Organism |
---|---|---|
arylsulfate sulfotransferase | - |
Desulfitobacterium hafniense |
AST | - |
Desulfitobacterium hafniense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
the melting temperature is at 47°C | Desulfitobacterium hafniense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
- |
Desulfitobacterium hafniense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Desulfitobacterium hafniense |