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Literature summary for 2.8.2.22 extracted from

  • Kim, D.H.; Kim, H.S.; Kobashi, K.
    Purification and characterization of novel sulfotransferase obtained from Klebsiella K-36, an intestinal bacterium of rat (1992), J. Biochem., 112, 456-460.
    View publication on PubMed
Search for more literature for 2.8.2.22

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 33% inhibition at 2 mM Klebsiella sp.
Cd2+ almost complete inhibition at 2 mM Klebsiella sp.
Cu2+ 37% inhibition at 2 mM Klebsiella sp.
EDTA inactivation reversed by addition of metal ions Klebsiella sp.
Ni2+ 32% inhibition at 2 mM Klebsiella sp.
Pb2+ 35% inhibition at 2 mM Klebsiella sp.
Zn2+ 38% inhibition at 2 mM Klebsiella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 4-nitrophenyl sulfate with phenol as second substrate, pH 8, 37°C Klebsiella sp.
0.66
-
 phenol with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C Klebsiella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 33% increase at 2 mM Klebsiella sp.
Mg2+ 65% increase at 2 mM Klebsiella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 2 * 73000, SDS-PAGE Klebsiella sp.
160000
-
 gel filtration Klebsiella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella sp. may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation ?
-
 ?
additional information Klebsiella sp. K-36 may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella sp.
-
 K-36
-
Klebsiella sp. K-36
-
 K-36
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.82
-
-
 Klebsiella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-acetylphenyl sulfate + phenol 28% of the activity with 4-nitrophenyl sulfate Klebsiella sp. 4-acetylphenol + phenyl sulfate
-
 ?
4-methylumbelliferyl sulfate + phenol 55% of the activity with p-nitrophenyl sulfate Klebsiella sp. 4-methylumbelliferol + phenyl sulfate
-
 ?
4-nitrophenyl sulfate + 3-chlorophenol
-
 Klebsiella sp. 3-chlorophenyl sulfate + 4-nitrophenol
-
 ?
4-nitrophenyl sulfate + 3-chlorophenol
-
 Klebsiella sp. K-36 3-chlorophenyl sulfate + 4-nitrophenol
-
 ?
4-nitrophenyl sulfate + 9-phenanthrol
-
 Klebsiella sp. 9-phenanthroyl sulfate + 4-nitrophenol
-
 ?
4-nitrophenyl sulfate + 9-phenanthrol
-
 Klebsiella sp. K-36 9-phenanthroyl sulfate + 4-nitrophenol
-
 ?
4-nitrophenyl sulfate + alpha-naphthol
-
 Klebsiella sp. 4-nitrophenol + 1-naphththyl sulfate
-
 ?
4-nitrophenyl sulfate + alpha-naphthol
-
 Klebsiella sp. K-36 4-nitrophenol + 1-naphththyl sulfate
-
 ?
4-nitrophenyl sulfate + phenol
-
 Klebsiella sp. phenyl sulfate + 4-nitrophenol
-
 ?
estrone sulfate + phenol 11% of the activity with p-nitrophenyl sulfate Klebsiella sp. estrone + phenyl sulfate
-
 ?
additional information may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation Klebsiella sp. ?
-
 ?
additional information may play a role in metabolism and detoxification of phenolic compounds, through the enzymatic sulfation Klebsiella sp. K-36 ?
-
 ?
phenyl sulfate + an aryl alcohol
-
 Klebsiella sp. phenol + an aryl sulfate
-
 ?
phenyl sulfate + an aryl alcohol
-
 Klebsiella sp. K-36 phenol + an aryl sulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 73000, SDS-PAGE Klebsiella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Klebsiella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable below Klebsiella sp.
60
-
 10 min, inactivation Klebsiella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 10.5
-
 Klebsiella sp.