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Literature summary for 2.8.2.20 extracted from

  • Kim, T.H.; Kim, D.H.; Nam, H.W.; Park, S.Y.; Shim, J.; Cho, J.W.
    Tyrosylprotein sulfotransferase regulates collagen secretion in Caenorhabditis elegans (2010), Mol. Cells, 29, 413-418.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus the enzyme is localized to the trans-Golgi network Caenorhabditis elegans 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine Caenorhabditis elegans the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine Caenorhabditis elegans N2 the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Caenorhabditis elegans N2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose bead chromatography, and gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro Caenorhabditis elegans adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro Caenorhabditis elegans N2 adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
TPST-1
-
Caenorhabditis elegans
tyrosylprotein sulfotransferase
-
Caenorhabditis elegans

General Information

General Information Comment Organism
malfunction knockdown of the enzyme blocks the cuticle localization of ROL-6 Caenorhabditis elegans
physiological function the enzyme regulates cuticle organization by promoting the transport of ROL-6 from the trans Golgi network to the cuticle. The sulfation of ROL-6 by TPST-1 is critical for its proper localization Caenorhabditis elegans