Cloned (Comment) | Organism |
---|---|
expressed in HEK-293 cells | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | the enzyme is localized to the trans-Golgi network | Caenorhabditis elegans | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine | Caenorhabditis elegans | the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro | adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine | Caenorhabditis elegans N2 | the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro | adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Caenorhabditis elegans N2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose bead chromatography, and gel filtration | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine | the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro | Caenorhabditis elegans | adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine | the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro | Caenorhabditis elegans N2 | adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
TPST-1 | - |
Caenorhabditis elegans |
tyrosylprotein sulfotransferase | - |
Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of the enzyme blocks the cuticle localization of ROL-6 | Caenorhabditis elegans |
physiological function | the enzyme regulates cuticle organization by promoting the transport of ROL-6 from the trans Golgi network to the cuticle. The sulfation of ROL-6 by TPST-1 is critical for its proper localization | Caenorhabditis elegans |