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Literature summary for 2.8.2.20 extracted from

  • Chen, B.H.; Wang, C.C.; Lu, L.Y.; Hung, K.S.; Yang, Y.S.
    Fluorescence assay for protein post-translational tyrosine sulfation (2013), Anal. Bioanal. Chem., 405, 1425-1429.
    View publication on PubMed
Search for more literature for 2.8.2.20

Cloned(Commentary)

Cloned (Comment) Organism
substance protein A-fused tyrosylprotein sulfotransferase is expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenylyl sulfate
-
 Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 3'-phosphoadenylyl sulfate using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.011
-
 P-selectin glycoprotein ligand-1 using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.016
-
 3'-phosphoadenylyl sulfate using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.053
-
 P-selectin glycoprotein ligand-1 using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 Drosophila melanogaster
-
 adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 Drosophila melanogaster i.e. ATEYEYLDYDFL adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
-
 Drosophila melanogaster adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
 ?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 i.e. ATEYEYLDYDFL Drosophila melanogaster adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
-
 ?

Synonyms

Synonyms Comment Organism
TPST
-
 Drosophila melanogaster
tyrosylprotein sulfotransferase
-
 Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
 P-selectin glycoprotein ligand-1 using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.058
-
 3'-phosphoadenylyl sulfate using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.1
-
 P-selectin glycoprotein ligand-1 using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.11
-
 3'-phosphoadenylyl sulfate using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.031
-
 3'-phosphoadenylyl sulfate using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
0.061
-
 3'-phosphoadenylyl sulfate using P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 P-selectin glycoprotein ligand-1 using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
4.2
-
 P-selectin glycoprotein ligand-1 using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
6.7
-
 3'-phosphoadenylyl sulfate using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster
18.2
-
 3'-phosphoadenylyl sulfate using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C Drosophila melanogaster