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Literature summary for 2.8.2.20 extracted from
- Conversion of recombinant hirudin to the natural form by in vitro tyrosine sulfation. Differential substrate specificities of leech and bovine tyrosylprotein sulfotransferases (1990), J. Biol. Chem., 265, 9314-9318.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
CCK-(107-115) | pH 6.5, 30°C | Hirudo medicinalis |  |
| 0.11 | - |
peptide CCK-(107-115) | pH 6.5, 30°C | Bos taurus | |
| 0.24 | - |
peptide Hir-(57-65) | pH 6.5, 30°C | Bos taurus | |
| 0.41 | - |
peptide Hir-(57-65) | pH 6.5, 30°C | Hirudo medicinalis | |
| 5.6 | - |
recombinant hirudin HV2 | pH 6.5, 30°C | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
| membrane | - |
Hirudo medicinalis | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Hirudo medicinalis | - |
leech | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal medulla | - |
Bos taurus | - |
| salivary gland | - |
Hirudo medicinalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + peptide Hir-(57-65) | - |
Bos taurus | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + peptide Hir-(57-65) | - |
Hirudo medicinalis | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + recombinant hirudin HV2 | - |
Bos taurus | ? | - |
? | |
| 3'-phosphoadenylylsulfate + KKSAEDYEYPSMG | - |
Bos taurus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + KKSAEDYEYPSMG | - |
Hirudo medicinalis | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + preprocholecystokinin | CCK-(107-115) corresponding to the carboxy-terminal sulfation sites of the protein | Bos taurus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + preprocholecystokinin | CCK-(107-115) corresponding to the carboxy-terminal sulfation sites of the protein | Hirudo medicinalis | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + recombinant hirudin variants | rHV2 and Hir-(57-65) corresponding to the nine C-terminal amino acids of hirudin | Bos taurus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + recombinant hirudin variants | rHV2 and Hir-(57-65) corresponding to the nine C-terminal amino acids of hirudin | Hirudo medicinalis | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + recombinant hirudin variants | sulfated at physiological sulfation site: Tyr-63 | Bos taurus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + recombinant hirudin variants | sulfated at physiological sulfation site: Tyr-63 | Hirudo medicinalis | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + synthetic peptides | - |
Bos taurus | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylylsulfate + synthetic peptides | structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins | Hirudo medicinalis | adenosine 3',5'-bisphosphate + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
| 30 | - |
assay at | Hirudo medicinalis |
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