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Literature summary for 2.8.2.20 extracted from
- Inhibition of tyrosylprotein sulfotransferase by sphingosine and its reversal by acidic phospholipids (1993), Biochemistry, 32, 1194-1198.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lysophosphatidylcholine | slight activation, 0.2 mM | Rattus norvegicus |  |
| oleic acid | activation, 0.2 mM | Rattus norvegicus | |
| phosphatidylinositol | slight activation, 0.2 mM | Rattus norvegicus | |
| phosphatidylserine | slight activation, 0.2 mM | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-erythro-sphinganine | less effective than sphingosine | Rattus norvegicus | |
| L-threo-sphinganine | - |
Rattus norvegicus | |
| phosphatidylcholine | weak | Rattus norvegicus | |
| psychosine | almost complete inhibition | Rattus norvegicus | |
| sphingomyelin | weak | Rattus norvegicus | |
| sphingosine | 50% inhibition at 0.15 mM, kinetics, enhanced by phosphatidylcholine or sphingomyelin. Phosphatidylinositol, phosphatidylserine or oleic acid reverses | Rattus norvegicus | |
| Stearylamine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
EAY | acidic amino acid polymer, pH 6.8, 37°C | Rattus norvegicus | |
| 0.00025 | - |
3'-phosphoadenylylsulfate | pH 6.8, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Rattus norvegicus | 139 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | requirement | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| salivary gland | submandibular | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + EAY | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + EAY(O-sulfate) | - |
? | |
| 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY | i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
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