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Literature summary for 2.8.2.2 extracted from
- Human cytosolic sulfotransferase 2B1: isoform expression, tissue specificity and subcellular localization (2006), J. Steroid Biochem. Mol. Biol., 102, 214-221.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure | Rattus norvegicus |
| SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure | Mus musculus |
| SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure, the two different isozymes are encoded by the gene SULT2B1 utilizing different start sites of transcription resulting in the incorporation of different first exons, SULT2B1a and SULT2B1b are 350 and 365 amino acids in length, respectively, and the last 342 amino acids are identical, expression of His-tagged or GST-tagged SULT2B1b in Escherichia coli, expression of SULT2B1a and SULT2B1b in COS-1 cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | isozyme SULT2B1b, T47D or MCF-7 breast cancer cells, in prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells isozyme SULT2B1b is expressed only in the cytosol | Homo sapiens | 5829 | - |
| additional information | a unique 3'-extension of SULT2B1b is required for nuclear localization in human BeWo placental choriocarcinoma cells, the nuclear localization is stimulated by forskolin treatment in BeWo cells and serine phosphorylation in the 3'-extension | Homo sapiens | - |
- |
| nucleus | - |
Rattus norvegicus | 5634 | - |
| nucleus | - |
Mus musculus | 5634 | - |
| nucleus | isozyme SULT2B1b, T47D or MCF-7 breast cancer cells, SULT2B1b is not tightly bound in the nuclei | Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? |  |
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Mus musculus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate | - |
? | |
| additional information | Homo sapiens | SULT2B1 isoforms show selectivity for the sulfation of 3beta-hydroxysteroids including dehydroepiandrosterone and pregnenolone, and demonstrate little activity with 3alpha-hydroxysteroids and estrogens, SULT2B1a possesses a higher pregnenolone sulfation activity than SULT2B1b whereas SULT2B1b has a higher cholesterol sulfation activity, SULT2B1b is also capable of conjugating several drugs and xenobiotics including genistein, tibolone, and raloxifene | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O00204 | isozymes SULT2B1a and SULT2B1b | - |
| Mus musculus | O35400 | isozymes SULT2B1a and SULT2B1b | - |
| Rattus norvegicus | Q29YR5 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | higher expression level of SULT2B1 | Mus musculus | - |
| breast | - |
Homo sapiens | - |
| bronchus | - |
Homo sapiens | - |
| intestine | lower expression level of SULT2B1 | Mus musculus | - |
| LNCaP cell | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| MCF-7 cell | - |
Homo sapiens | - |
| additional information | no expression of SULT2B1b in alveolar tissue and in liver cytosol | Homo sapiens | - |
| additional information | no expression of SULT2B1b in placental, prostate and breast tissues in rodents | Rattus norvegicus | - |
| additional information | no expression of SULT2B1b in placental, prostate and breast tissues in rodents | Mus musculus | - |
| placenta | - |
Homo sapiens | - |
| prostate adenocarcinoma cell | - |
Homo sapiens | - |
| prostate gland | prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells | Homo sapiens | - |
| skin | SULT2B1b, but not SULT2B1a | Homo sapiens | - |
| spinal cord | higher expression level of SULT2B1 | Mus musculus | - |
| T-47D cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + cholesterol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Mus musculus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + genistein | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl sulfate + pregnenolone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + raloxifene | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| 3'-phosphoadenylyl sulfate + tibolone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
| additional information | SULT2B1 isoforms show selectivity for the sulfation of 3beta-hydroxysteroids including dehydroepiandrosterone and pregnenolone, and demonstrate little activity with 3alpha-hydroxysteroids and estrogens, SULT2B1a possesses a higher pregnenolone sulfation activity than SULT2B1b whereas SULT2B1b has a higher cholesterol sulfation activity, SULT2B1b is also capable of conjugating several drugs and xenobiotics including genistein, tibolone, and raloxifene | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | SULT2B1 isoforms possess a 50 amino acid N-terminal extension that is enriched in serine and proline residues | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the SULT2 family | Homo sapiens |
| More | the enzyme belongs to the SULT2 family | Rattus norvegicus |
| More | the enzyme belongs to the SULT2 family | Mus musculus |
| SULT2B1 | - |
Homo sapiens |
| SULT2B1 | - |
Rattus norvegicus |
| SULT2B1 | - |
Mus musculus |
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