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Literature summary for 2.8.2.2 extracted from

  • Fuda, H.; Javitt, N.B.; Mitamura, K.; Ikegawa, S.; Strott, C.A.
    Oxysterols are substrates for cholesterol sulfotransferase (2007), J. Lipid Res., 48, 1343-1352.
    View publication on PubMed
Search for more literature for 2.8.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged SULT2B1 Homo sapiens
expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + 7-oxocholesterol Homo sapiens SULT2B1b, SULT2B1a, and SULT2A1 adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + cholesterol Homo sapiens SULT2B1b, SULT2B1a, and SULT2A1 adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone Homo sapiens SULT2A1, low activity with SULT2B1b and SULT2B1a adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens O00204
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged SULT2B1 by glutathione affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
atheroma cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
additional information no expression in 293T cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0019
-
 recombinant SULT2B1b, substrate 7beta-hydroxycholesterol Homo sapiens
0.0048
-
 recombinant SULT2B1b, substrate cholesterol 7alpha-hydroperoxide Homo sapiens
0.0071
-
 recombinant SULT2B1b, substrate 7-oxocholesterol Homo sapiens
0.0075
-
 recombinant SULT2B1b, substrate cholesterol Homo sapiens
0.021
-
 recombinant SULT2B1b, substrate 7alpha-hydroxycholesterol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate
-
 ?
3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate
-
 ?
3'-phosphoadenylyl sulfate + 7-oxo-cholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + 7-oxocholesterol SULT2B1b, SULT2B1a, and SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol SULT2B1b, the 7alpha derivative is highly preferred Homo sapiens adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol SULT2B1b, the 7alpha derivative is highly preferred Homo sapiens adenosine 3',5'-bisphosphate + 7beta-hydroxycholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + cholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + cholesterol SULT2B1b, SULT2B1a, and SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + cholesterol 7alpha-hydroperoxide SULT2B1b Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone SULT2A1, low activity with SULT2B1b and SULT2B1a Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
additional information SULT2B1b sulfates oxosterols, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cholesterol sulfotransferase
-
 Homo sapiens
More cf. EC 2.8.2.15 Homo sapiens
steroid/sterol sulfotransferase
-
 Homo sapiens
SULT2A1
-
 Homo sapiens
SULT2B1a
-
 Homo sapiens
SULT2B1b
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.5 assay at Homo sapiens