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Literature summary for 2.8.2.2 extracted from

  • Sheng, J.J.; Duffel, M.W.
    Bacterial expression, purification, and characterization of rat hydroxysteroid sulfotransferase STa (2001), Protein Expr. Purif., 21, 235-242.
    View publication on PubMed
Search for more literature for 2.8.2.2

Application

Application Comment Organism
medicine model studies on the carcinogenesis of benzylic and allylic alcohols Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
STa cloned into pET-3c vector and expressed in Escherichia coli BL21 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
dehydroepiandrosterone substrate inhibition Rattus norvegicus
S-(-)-1-naphthyl-1-ethanol
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 (R)-(+)-1-phenyl-1-heptanol pH 7.0, 37°C Rattus norvegicus
0.31
-
 (S)-(-)-1-phenyl-1-heptanol pH 7.0, 37°C Rattus norvegicus
0.72
-
 7-hydroxymethyl-12-methylbenz[a]anthracene pH 7.0, 37°C Rattus norvegicus
0.96
-
 (R)-(+)-1-naphthyl-1-ethanol pH 7.0, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33600
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P22789
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant protein Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + (R)-(+)-1-phenyl-1-heptanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + (R)-(+)-1-phenyl-1-heptyl sulfate
-
 ?
3'-phosphoadenylylsulfate + (S)-(-)-1-phenyl-1-heptanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + (S)-(-)-1-phenyl-1-heptyl sulfate
-
 r
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 7-hydroxymethyl-12-methylbenz[a]anthracene sulfate
-
 ?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
additional information (S)-(-)-1-naphthyl-1-ethanol is not a substrate for STa Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
STA
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
 (S)-(-)-1-naphthyl-1-ethanol pH 5.5, 37°C Rattus norvegicus