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Literature summary for 2.8.2.2 extracted from

  • Rearick, J.I.; Calhoun, E.S.
    Purification and characterization of cholesterol sulfotransferase from rat skin (2001), Biochem. Cell Biol., 79, 499-506.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information stimulated by nonionic detergents Rattus norvegicus

General Stability

General Stability Organism
partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3',5'-bisphosphate competitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00063
-
3beta,20beta-pregnenediol pH 7.5, 37°C Rattus norvegicus
0.0016
-
pregnenolone pH 7.5, 37°C Rattus norvegicus
0.0025
-
cholesterol pH 7.5, 37°C Rattus norvegicus
0.0086
-
dehydroepiandrosterone pH 7.5, 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Rattus norvegicus
50000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
epidermis epidermal keratinocyte Rattus norvegicus
-
epithelium
-
Rattus norvegicus
-
skin
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0084
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight Rattus norvegicus
4°C, partially purified enzyme loses 30% of activity overnight Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + 3beta,20beta-pregnenediol
-
Rattus norvegicus ?
-
?
3'-phosphoadenylylsulfate + cholesterol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + cholesterol sulfate
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + (3alpha,20S)-20-hydroxypregn-5-en-3-yl sulfate
-
r
3'-phosphoadenylylsulfate + pregnenolone
-
Rattus norvegicus adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
-
r
additional information 3beta-hydroxysteroids with a DELTA5 double bond serve as substrates while androgens, estrogens, corticosteroids, p-nitrophenol and 3,4-dihydroxyphenylalanine do not serve as substrates Rattus norvegicus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
broad pH optimum centered on pH 8.0 Rattus norvegicus