Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | stimulated by nonionic detergents | Rattus norvegicus |
General Stability | Organism |
---|---|
partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-bisphosphate | competitive inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00063 | - |
3beta,20beta-pregnenediol | pH 7.5, 37°C | Rattus norvegicus | |
0.0016 | - |
pregnenolone | pH 7.5, 37°C | Rattus norvegicus | |
0.0025 | - |
cholesterol | pH 7.5, 37°C | Rattus norvegicus | |
0.0086 | - |
dehydroepiandrosterone | pH 7.5, 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Rattus norvegicus |
50000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | epidermal keratinocyte | Rattus norvegicus | - |
epithelium | - |
Rattus norvegicus | - |
skin | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0084 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight | Rattus norvegicus |
4°C, partially purified enzyme loses 30% of activity overnight | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 3beta,20beta-pregnenediol | - |
Rattus norvegicus | ? | - |
? | |
3'-phosphoadenylylsulfate + cholesterol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cholesterol sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + (3alpha,20S)-20-hydroxypregn-5-en-3-yl sulfate | - |
r | |
3'-phosphoadenylylsulfate + pregnenolone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate | - |
r | |
additional information | 3beta-hydroxysteroids with a DELTA5 double bond serve as substrates while androgens, estrogens, corticosteroids, p-nitrophenol and 3,4-dihydroxyphenylalanine do not serve as substrates | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad pH optimum centered on pH 8.0 | Rattus norvegicus |