Literature summary for 2.8.2.2 extracted from

Li, X.; Anderson, R.J.
Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases (1999), Biochem. Biophys. Res. Commun., 263, 632-639.

Search for more literature for 2.8.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
DHEA ST and SULT2A1 cloned and expressed in COS-1 cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00136	-	tetraiodothyronine	pH 6.0, 37°C, isoform SULT1E1	Homo sapiens
0.00295	-	3,3'-diiodothyronine	pH 6.0, 37°C, isoform SULT2A1	Homo sapiens
0.00463	-	triiodothyronine	pH 6.0, 37°C, recombinant isoform SULT1E1	Homo sapiens
0.00484	-	3,5-diiodothyronine	pH 6.0, 37°C, isoform SULT2A1	Homo sapiens
0.00607	-	triiodothyronine	pH 6.0, 37°C, isoform SULT1E1	Homo sapiens
0.00704	-	triiodothyronine	pH 6.0, 37°C, recombinant isoform SULT2A1	Homo sapiens
0.00973	-	3,3'-diiodothyronine	pH 6.0, 37°C, isoform SULT1E1	Homo sapiens
0.0143	-	triiodothyronine	pH 6.0, 37°C, isoform SULT2A1	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone	Homo sapiens	sulfation is an important pathway in the metabolism of thyroid hormones, sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal fetal circulation	adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	human	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylylsulfate + 3,3'-diiodothyronine	sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + 3,5-diiodothyronine	sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + dehydroepiandrosterone	-	Homo sapiens	adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate	-	r
3'-phosphoadenylylsulfate + dehydroepiandrosterone	sulfation is an important pathway in the metabolism of thyroid hormones, sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal fetal circulation	Homo sapiens	adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate	-	r
3'-phosphoadenylylsulfate + diiodothyronine	sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + tetraiodothyronine	sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?
3'-phosphoadenylylsulfate + triiodothyronine	sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group	Homo sapiens	adenosine 3',5'-bisphosphate + ?	-	?

Synonyms

Synonyms	Comment	Organism
DHEA-ST	-	Homo sapiens
hydroxysteroid sulfotransferase ST2A3	also variously named as SULT2A1 or human DHEA-ST	Homo sapiens
iodothyronine sulfotransferase	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	43	50% inactivation temperature for SULT1E1 41.0°C, for SULT2A1 45.5°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	isoform SULT2A1	Homo sapiens

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Release 2023.1 (January 2023)